PMID- 19142972
OWN - NLM
STAT- MEDLINE
DCOM- 20090526
LR  - 20090319
IS  - 1096-9896 (Electronic)
IS  - 0022-3417 (Linking)
VI  - 217
IP  - 5
DP  - 2009 Apr
TI  - Induction of anti-tumour immunity by dendritic cells transduced with hTERT 
      recombinant adenovirus in mice.
PG  - 685-92
LID - 10.1002/path.2493 [doi]
AB  - Dendritic cells (DCs) transfected with recombinant, replication-defective 
      adenovirus (Ad) vectors encoding the human telomerase reverse transcriptase 
      (hTERT) are potent inducers of cytotoxic T lymphocytes (CTLs) and anti-tumour 
      immunity. However, previous studies have mostly been in vitro. In this study, we 
      sought to determine whether DCs transfected with hTERT (DC/Ad-hTERT) could elicit 
      a potent anti-tumour immunogenic response in vivo. We found that murine DCs 
      transfected with recombinant adenovirus encoding the hTERT gene (DC/Ad-hTERT) 
      induced hTERT-specific CTLs in vivo effectively, compared with Ad-LacZ-transduced 
      DC (DC/Ad-LacZ) controls. These hTERT-specific CTLs lysed various tumour cell 
      lines in an hTERT-specific and MHC-I molecule-restricted fashion. We also found 
      that DC/Ad-hTERT could increase antigen-specific T-cell proliferation and augment 
      the number of IFN-gamma secreting T-cells in mice. These data suggest that the 
      DC/Ad-hTERT vaccine may induce anti-tumour immunity against tumour cells 
      expressing hTERT in an MHC-I molecule-restricted fashion in vivo through the 
      augmentation of the hTERT-specific CTL response. The DC/Ad-hTERT vaccine may thus 
      be used as an efficient DC-based tumour vaccine in clinical applications.
FAU - Chen, Ling
AU  - Chen L
AD  - Institute of Gastroenterology of PLA, Southwest Hospital, Third Military Medical 
      University, Chongqing 400038, China.
FAU - Tang, Xu-Dong
AU  - Tang XD
FAU - Yu, Song-Tao
AU  - Yu ST
FAU - Ai, Zhi-Hua
AU  - Ai ZH
FAU - Fang, Dian-Chun
AU  - Fang DC
FAU - Cai, Yong-Guo
AU  - Cai YG
FAU - Luo, Yuan-Hui
AU  - Luo YH
FAU - Liang, Guang-Ping
AU  - Liang GP
FAU - Yang, Shi-Ming
AU  - Yang SM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (Cancer Vaccines)
RN  - 0 (HLA-A2 Antigen)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.7.7.49 (TERT protein, human)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Cancer Vaccines/*immunology
MH  - Cytotoxicity, Immunologic/immunology
MH  - Dendritic Cells/*immunology/transplantation
MH  - Female
MH  - Genetic Vectors
MH  - HLA-A2 Antigen/metabolism
MH  - Immunophenotyping
MH  - Interferon-gamma/biosynthesis
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Telomerase/genetics/*immunology
MH  - Transduction, Genetic
MH  - Tumor Cells, Cultured
EDAT- 2009/01/15 09:00
MHDA- 2009/05/27 09:00
CRDT- 2009/01/15 09:00
PHST- 2009/01/15 09:00 [entrez]
PHST- 2009/01/15 09:00 [pubmed]
PHST- 2009/05/27 09:00 [medline]
AID - 10.1002/path.2493 [doi]
PST - ppublish
SO  - J Pathol. 2009 Apr;217(5):685-92. doi: 10.1002/path.2493.