PMID- 19143400
OWN - NLM
STAT- MEDLINE
DCOM- 20091029
LR  - 20161018
IS  - 0250-3301 (Print)
IS  - 0250-3301 (Linking)
VI  - 29
IP  - 10
DP  - 2008 Oct
TI  - [Microbial community structure of beer wastewater treatment plants analyzed by 
      PCR-DGGE technique].
PG  - 2950-5
AB  - The microbial community structure of beer wastewater treatment plants with 
      hydrolyze-acidification and SBR process was studied by PCR amplification and 
      denaturing gradient gel electrophoresis (DGGE) based 16S rDNA. Activated sludge 
      samples were collected from both hydrolyze-acidification tank and SBR tank at 
      different depth and disposal period. The total DNA was extracted and the 16S rDNA 
      was amplified by universal primer. The microbial community structure was analyzed 
      by denaturing gradient gel electrophoresis, compared with the DGGE band pattern 
      of the screened high efficient bacteria. The PCR products were cloned and 
      sequenced to analyze the microbial community in phylogenic evolution. The DGGE 
      pattern showed that the microbial community in hydrolyze-acidification tank 
      changed with the variation of depth both in microbial structure and population, 
      and at the depth of two-meter the microbial community was relatively rich, the 
      dominant bands were notable. The microbial community in SBR tank at different 
      depths and different disposal periods (precipitation period, inflow period, 
      aeration period) were consistent respectively, but the dominant bands were 
      different. Sequences of y2, 23, 25, 31, h5, 15 yielded high homogeneity with the 
      strains uncultured Thermotogales sp., Comamonas sp. WT OTU1, Agrobacterium 
      tumefaciens, Bacillus subtilis, Bdellovibrio bacteriovorus, Comamonas 
      testosteroni from GenBank. The 16S rDNA sequences of the dominant bands were not 
      completely same with that of the high efficient bacteria, which indicated that 
      the strains screened were not the dominant strains in actual process.
FAU - Zhao, Ji-Hong
AU  - Zhao JH
AD  - School of Chemistry and Chemical Engineering, Henan University of Technology, 
      Zhengzhou 450001, China. zhaojihong@haut.edu.cn
FAU - He, Shu-Ying
AU  - He SY
FAU - Li, Ji-Xiang
AU  - Li JX
FAU - Liu, Yong-De
AU  - Liu YD
FAU - Lou, Yan
AU  - Lou Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Huan Jing Ke Xue
JT  - Huan jing ke xue= Huanjing kexue
JID - 8405344
SB  - IM
MH  - *Beer
MH  - Bioreactors/*microbiology
MH  - Comamonas/genetics/*isolation & purification
MH  - Electrophoresis, Gel, Pulsed-Field/methods
MH  - Polymerase Chain Reaction/methods
MH  - Rhizobium/genetics/isolation & purification
MH  - Thermotoga maritima/genetics/*isolation & purification
MH  - Waste Disposal, Fluid/*methods
EDAT- 2009/01/16 09:00
MHDA- 2009/10/30 06:00
CRDT- 2009/01/16 09:00
PHST- 2009/01/16 09:00 [entrez]
PHST- 2009/01/16 09:00 [pubmed]
PHST- 2009/10/30 06:00 [medline]
PST - ppublish
SO  - Huan Jing Ke Xue. 2008 Oct;29(10):2950-5.