PMID- 19144013
OWN - NLM
STAT- MEDLINE
DCOM- 20100426
LR  - 20100208
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 45
IP  - 1
DP  - 2010 Feb
TI  - Effects of serum-free in vitro maturation of bovine oocytes on subsequent embryo 
      development and cell allocation in two developmental stages of day 7 blastocysts.
PG  - 42-9
LID - 10.1111/j.1439-0531.2008.01203.x [doi]
AB  - Maturation of oocytes and the subsequent outcome of the in vitro production (IVP) 
      are affected by the composition of in vitro maturation (IVM) medium. To determine 
      the use of serum interfering with effects of single molecules, we aimed at 
      developing simplified IVM medium. The experimental IVM media were: (1) 
      M199-medium supplemented with hormones and serum (control), (2) as 1 but serum 
      was substituted with fatty acid-free serum albumin (FAFBSA) and (3) M199-medium 
      without hormonal and serum supplementation (M199). The quality of embryos was 
      assessed on day 7 by morphology and cryotolerance, as well as by Terminal 
      deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling (TUNEL) and 
      differential staining. Results showed that the nuclear maturation was suppressed 
      in M199 group alone. Embryo cleavage and development rates, and the proportion of 
      quality 1 blastocysts were lower in the FAFBSA and M199 groups compared to the 
      control. Differences in the cell allocation of fresh embryos were observed at the 
      blastocyst stage, but not at the expanded blastocyst stage. The control group 
      blastocysts had larger number of cells allocated to the inner cell mass (ICM), 
      and the FAFBSA group blastocysts larger apoptotic cell proportion compared to the 
      blastocysts derived from other groups. After cryopreservation, the reduction of 
      ICM proportion and increase of apoptotic cell proportion of embryos were equal 
      between the experimental groups. In conclusion, exclusion of serum from the IVM 
      media reduces embryo development and may cause perturbations in blastocyst 
      development. Differences in the cell allocation of blastocysts between IVM media 
      may appear only when the developmental stages are taken into account.
FAU - Korhonen, K
AU  - Korhonen K
AD  - Biotechnology and Food Research, Animal Genomics, MTT Agrifood Research Finland, 
      Jokioinen, Finland.
FAU - Kananen, K
AU  - Kananen K
FAU - Ketoja, E
AU  - Ketoja E
FAU - Matomaki, J
AU  - Matomaki J
FAU - Halmekyto, M
AU  - Halmekyto M
FAU - Peippo, J
AU  - Peippo J
LA  - eng
PT  - Journal Article
DEP - 20081230
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
RN  - 0 (Culture Media, Serum-Free)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Blastocyst/*cytology/physiology
MH  - *Cattle/embryology
MH  - Cell Nucleus/physiology
MH  - Cells, Cultured
MH  - Cleavage Stage, Ovum/physiology
MH  - Cryopreservation/veterinary
MH  - *Culture Media, Serum-Free
MH  - Embryonic Development/*physiology
MH  - Female
MH  - In Situ Nick-End Labeling
MH  - Oocytes/*growth & development/ultrastructure
EDAT- 2009/01/16 09:00
MHDA- 2010/04/27 06:00
CRDT- 2009/01/16 09:00
PHST- 2009/01/16 09:00 [entrez]
PHST- 2009/01/16 09:00 [pubmed]
PHST- 2010/04/27 06:00 [medline]
AID - RDA1203 [pii]
AID - 10.1111/j.1439-0531.2008.01203.x [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2010 Feb;45(1):42-9. doi: 10.1111/j.1439-0531.2008.01203.x. 
      Epub 2008 Dec 30.