PMID- 19144080
OWN - NLM
STAT- MEDLINE
DCOM- 20090223
LR  - 20181201
IS  - 1365-3083 (Electronic)
IS  - 0300-9475 (Linking)
VI  - 69
IP  - 2
DP  - 2009 Feb
TI  - Inhibition of human neutrophil apoptosis by Paracoccidioides brasiliensis: role 
      of interleukin-8.
PG  - 73-9
LID - 10.1111/j.1365-3083.2008.02199.x [doi]
AB  - Paracoccidioidomycosis (PCM) is a systemic mycosis caused by Paracoccidiodes 
      brasiliensis that presents a wide spectrum of clinical manifestations. Because of 
      the great number of neutrophils polymorphonuclear neutrophils (PMN) found in the 
      P. brasiliensis granuloma, studies have been done to evaluate the role of these 
      cells during the development of the infection. This fungus is found 
      intracellularly in PMN and monocytes/macrophages, suggesting that it is capable 
      of evading damage and surviving inside these cells. Thus, in the present study, 
      we investigated whether P. brasiliensis can prolong the lifetime of PMN, and if 
      this process would be related with IL-8 levels. PMN apoptosis and intracellular 
      levels of IL-8 were analysed by flow cytometry and culture supernatants IL-8 
      levels were evaluated by enzyme-linked immunosorbent assay. We found that 
      coincubation with P. brasiliensis yeast cells results in an inhibition of PMN 
      apoptosis, which was associated with increase in IL-8 production by these cells. 
      Cocultures treatment with monoclonal antibody anti-IL-8 reversed the inhibitory 
      effect of P. brasiliensis on PMN apoptosis, besides to increase spontaneous 
      apoptosis of these cells. These data show that, in contrast to other microbial 
      pathogens that drive phagocytes into apoptosis to escape killing, P. brasiliensis 
      can extend the lifetime of normal human PMN by inducing autocrine IL-8 
      production.
FAU - Acorci, M J
AU  - Acorci MJ
AD  - Department of Microbiology and Immunology, Biosciences Institute, Sao Paulo State 
      University, Botucatu, SP, Brazil.
FAU - Dias-Melicio, L A
AU  - Dias-Melicio LA
FAU - Golim, M A
AU  - Golim MA
FAU - Bordon-Graciani, A P
AU  - Bordon-Graciani AP
FAU - Peracoli, M T S
AU  - Peracoli MT
FAU - Soares, A M V C
AU  - Soares AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081210
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Interleukin-8)
SB  - IM
MH  - Adult
MH  - Antibodies, Monoclonal/immunology
MH  - *Apoptosis
MH  - Humans
MH  - Interleukin-8/metabolism/*physiology
MH  - Middle Aged
MH  - Neutrophils/*physiology
MH  - Paracoccidioides/*physiology
MH  - Phagocytosis
EDAT- 2009/01/16 09:00
MHDA- 2009/02/24 09:00
CRDT- 2009/01/16 09:00
PHST- 2009/01/16 09:00 [entrez]
PHST- 2009/01/16 09:00 [pubmed]
PHST- 2009/02/24 09:00 [medline]
AID - SJI2199 [pii]
AID - 10.1111/j.1365-3083.2008.02199.x [doi]
PST - ppublish
SO  - Scand J Immunol. 2009 Feb;69(2):73-9. doi: 10.1111/j.1365-3083.2008.02199.x. Epub 
      2008 Dec 10.