PMID- 19144870
OWN - NLM
STAT- MEDLINE
DCOM- 20090401
LR  - 20101118
IS  - 1535-3702 (Print)
IS  - 1535-3699 (Linking)
VI  - 234
IP  - 3
DP  - 2009 Mar
TI  - Investigation of the dermal sensitizing potential of traditional medical extracts 
      in local lymph node assays.
PG  - 306-13
LID - 10.3181/0809-RM-281 [doi]
AB  - Traditional medical extracts are commonly used as complex mixtures, which may 
      contain naturally occurring contact sensitizers. In this investigation, the mice 
      local lymph node assay (LLNA) was performed to evaluate the dermal sensitization 
      potential of Myrrh, Borneolum, Olibanum, Moschus and Cassia Bark, which are 
      widely used in topical traditional medication. In the radioactive LLNA, the 
      stimulation index (SI) values were calculated for each medical extract. Myrrh, 
      Borneolum, Olibanum and Moschus induced dose-dependent cell proliferation and SI 
      was more than 3. Cassia Bark showed no positive response over the range of test 
      concentrations. In the flow cytometry analysis, the total number of CD3(+), 
      CD4(+), and CD8(+) cells in local lymph nodes was increased in Moschus-, 
      Olibanum-, Myrrh- and Borneolum-treated mice. The ratio of the B220(+)/CD3(+) 
      (B/T cell ratio) and the percentage of I-A(k+) cells that was also positive for 
      the CD69 marker (I-A(k+)/ CD69(+)) were increased in the Moschus-, Olibanum- and 
      Myrrh-treated mice. However, no ofbvious change was observed in Borneolum-treated 
      mice. Cassia Bark did not induce changes in the lymphocyte subpopulations. These 
      results indicate that Moschus, Olibanum and Myrrh can be regarded as sensitizers, 
      and Borneolum regarded as an irritant. Cassia Bark is neither a sensitizer nor an 
      irritant. The combination of radioactive and flow cytometric LLNA can be used for 
      the prediction of sensitizing potential of medical extracts which lead to 
      allergic contact dermatitis in humans.
FAU - Lv, Lei
AU  - Lv L
AD  - National Chengdu Center for Safety Evaluation of Drugs, West China Hospital of 
      Sichuan University, Chengdu 610041, China.
FAU - Yan, Guang-Yan
AU  - Yan GY
FAU - Zhao, Ying-Lan
AU  - Zhao YL
FAU - He, Xiao-Juan
AU  - He XJ
FAU - Jiang, Xing
AU  - Jiang X
FAU - Zhuo, Yan-Qiang
AU  - Zhuo YQ
FAU - Wang, Yan-Li
AU  - Wang YL
FAU - Wang, Li
AU  - Wang L
FAU - Cen, Xiao-Bo
AU  - Cen XB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090114
PL  - Switzerland
TA  - Exp Biol Med (Maywood)
JT  - Experimental biology and medicine (Maywood, N.J.)
JID - 100973463
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (CD69 antigen)
RN  - 0 (Irritants)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Antigens, Differentiation, T-Lymphocyte/metabolism
MH  - Cell Proliferation/drug effects
MH  - Dermis/*drug effects/*immunology
MH  - Ear/anatomy & histology
MH  - Flow Cytometry
MH  - Irritants/*pharmacology
MH  - Lectins, C-Type
MH  - *Local Lymph Node Assay
MH  - Lymph Nodes/cytology/drug effects
MH  - Lymphocytes/cytology/drug effects
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Organ Size/drug effects
MH  - Plant Extracts/*pharmacology
MH  - Plants, Medicinal/*metabolism
EDAT- 2009/01/16 09:00
MHDA- 2009/04/02 09:00
CRDT- 2009/01/16 09:00
PHST- 2009/01/16 09:00 [entrez]
PHST- 2009/01/16 09:00 [pubmed]
PHST- 2009/04/02 09:00 [medline]
AID - 0809-RM-281 [pii]
AID - 10.3181/0809-RM-281 [doi]
PST - ppublish
SO  - Exp Biol Med (Maywood). 2009 Mar;234(3):306-13. doi: 10.3181/0809-RM-281. Epub 
      2009 Jan 14.