PMID- 19146941
OWN - NLM
STAT- MEDLINE
DCOM- 20090609
LR  - 20220330
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 122
IP  - 1
DP  - 2009 Feb 25
TI  - Anti-inflammatory activity of a water extract of Acorus calamus L. leaves on 
      keratinocyte HaCaT cells.
PG  - 149-56
LID - 10.1016/j.jep.2008.12.011 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Acorus calamus L., sweet flag, is a well-known 
      medicinal plant that grows worldwide wildly along swamps, rivers, and lakes. AIM 
      OF THE STUDY: The aim of this study was to evaluate the anti-inflammatory 
      activity of Acorus calamus leaf (ACL) extract and to explore its mechanism of 
      action on human keratinocyte HaCaT cells. MATERIALS AND METHODS: HaCaT cells 
      treated with polyinosinic:polycytidylic acid (polyI:C) and peptidoglycan (PGN) 
      induced the inflammatory reactions. The anti-inflammatory activities of ACL were 
      investigated using RT-PCR, ELISA assay, immunoblotting, and immunofluorescence 
      staining. RESULTS: HaCaT cells induced the pro-inflammatory cytokines, 
      interleukin-8 (IL-8) and/or interleukin-6 (IL-6) expressions after treatment with 
      polyI:C or PGN. ACL inhibited the expression of IL-8 and IL-6 RNA and protein 
      levels, and attenuated the activation of NF-kappaB and IRF3 after polyI:C 
      treatment. ACL also inhibited expression of IL-8 and activation of NF-kappaB 
      following PGN induction. CONCLUSIONS: These results suggest that ACL inhibits the 
      production of pro-inflammatory cytokines through multiple mechanisms and may be a 
      novel and effective anti-inflammatory agent for the treatment of skin diseases.
FAU - Kim, Hyeri
AU  - Kim H
AD  - Department of Biotechnology, BK21 Graduate Program for Biotechnology, Chonnam 
      National University, 300 Yongbong-Dong, Buk-Gu, Gwangju 500-757, Republic of 
      Korea.
FAU - Han, Tae-Ho
AU  - Han TH
FAU - Lee, Seong-Gene
AU  - Lee SG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081225
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Interferon Regulatory Factor-3)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (NF-kappa B)
RN  - 0 (Peptidoglycan)
RN  - 0 (Plant Extracts)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - *Acorus
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Biological Transport/drug effects
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Humans
MH  - Inflammation/chemically induced/drug therapy
MH  - Interferon Regulatory Factor-3/antagonists & inhibitors
MH  - Interleukin-6/antagonists & inhibitors
MH  - Interleukin-8/antagonists & inhibitors
MH  - Keratinocytes/cytology/*drug effects
MH  - NF-kappa B/antagonists & inhibitors
MH  - Peptidoglycan/adverse effects
MH  - Phytotherapy
MH  - Plant Extracts/*pharmacology
MH  - Plant Leaves
MH  - Poly I-C/adverse effects
EDAT- 2009/01/17 09:00
MHDA- 2009/06/10 09:00
CRDT- 2009/01/17 09:00
PHST- 2008/09/24 00:00 [received]
PHST- 2008/11/18 00:00 [revised]
PHST- 2008/12/16 00:00 [accepted]
PHST- 2009/01/17 09:00 [entrez]
PHST- 2009/01/17 09:00 [pubmed]
PHST- 2009/06/10 09:00 [medline]
AID - S0378-8741(08)00703-4 [pii]
AID - 10.1016/j.jep.2008.12.011 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2009 Feb 25;122(1):149-56. doi: 10.1016/j.jep.2008.12.011. Epub 
      2008 Dec 25.