PMID- 19148127
OWN - NLM
STAT- MEDLINE
DCOM- 20090604
LR  - 20220331
IS  - 1930-7381 (Print)
IS  - 1930-7381 (Linking)
VI  - 17
IP  - 4
DP  - 2009 Apr
TI  - Innate immunity and adipocyte function: ligand-specific activation of multiple 
      Toll-like receptors modulates cytokine, adipokine, and chemokine secretion in 
      adipocytes.
PG  - 648-56
LID - 10.1038/oby.2008.607 [doi]
AB  - The aim of this study was to analyze Toll-like receptor (TLR) expression in 
      preadipocytes and mature adipocytes and to investigate whether TLR ligands 
      influence the release of cytokines, chemokines, and adipokines. Murine 3T3-L1 
      preadipocytes and mature adipocytes were used for stimulation experiments. The 
      effects of lipopolysaccharide (LPS), flagellin, Poly (U), Poly (I:C), 
      macrophage-activating lipopeptide-2 (MALP2), Pam3Cys, and CpG on the release of 
      interleukin-6 (IL-6), resistin, and monocyte chemoattractant protein-1 (MCP-1) 
      were determined by enzyme-linked immunosorbent assay (ELISA). Nuclear 
      translocation and promoter binding of NFkappaB were analyzed by electrophoretic 
      mobility shift assays. TLR expression was investigated by reverse-transcriptase 
      (RT-PCR). All TLRs except TLR5 and TRL7 are expressed in the stromal vascular 
      cell (SVC) fraction and in mature adipocytes of different fat stores. Whereas 
      basal and LPS-induced IL-6 release is higher in preadipocytes, basal and 
      LPS-induced MCP-1 release is higher in mature adipocytes. Mature adipocytes 
      respond to corticosterone regarding MCP-1 and resistin release. The ligands for 
      TLRs influence IL-6, MCP-1, and resistin release differentially. Some of these 
      ligands induce nuclear translocation and promoter binding of NFkappaB. Besides 
      TLR5, that is not expressed in mature adipocytes, all TLR family members are 
      involved. There exists a functional TRL pathway in adipocytes that connects 
      innate immunity with adipocyte function. As a consequence, the role of the 
      adipose tissue in both immunity and metabolism has to be investigated in future 
      studies. The results of this approach will help to explain the metabolic changes 
      such as insulin resistance observed during infection and the immunological 
      phenomena such as macrophage infiltration of adipose tissue seen in obesity.
FAU - Kopp, Andrea
AU  - Kopp A
AD  - Department of Internal Medicine I, Regensburg University Hospital, Regensburg, 
      Germany.
FAU - Buechler, Christa
AU  - Buechler C
FAU - Neumeier, Markus
AU  - Neumeier M
FAU - Weigert, Johanna
AU  - Weigert J
FAU - Aslanidis, Charalampos
AU  - Aslanidis C
FAU - Scholmerich, Juergen
AU  - Scholmerich J
FAU - Schaffler, Andreas
AU  - Schaffler A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090115
PL  - United States
TA  - Obesity (Silver Spring)
JT  - Obesity (Silver Spring, Md.)
JID - 101264860
RN  - 0 (Adipokines)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (Ligands)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Resistin)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptors)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Adipocytes/cytology/drug effects/*physiology
MH  - Adipokines/*metabolism
MH  - Animals
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Chemokines/*metabolism
MH  - Corticosterone/pharmacology
MH  - Cytokines/*metabolism
MH  - Humans
MH  - Immunity, Innate/*physiology
MH  - Interleukin-6/metabolism
MH  - Intra-Abdominal Fat/cytology/drug effects/physiology
MH  - Ligands
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - NF-kappa B/physiology
MH  - Resistin/metabolism
MH  - Signal Transduction/physiology
MH  - Subcutaneous Fat/cytology/drug effects/physiology
MH  - Toll-Like Receptor 4/physiology
MH  - Toll-Like Receptors/*physiology
EDAT- 2009/01/17 09:00
MHDA- 2009/06/06 09:00
CRDT- 2009/01/17 09:00
PHST- 2009/01/17 09:00 [entrez]
PHST- 2009/01/17 09:00 [pubmed]
PHST- 2009/06/06 09:00 [medline]
AID - oby2008607 [pii]
AID - 10.1038/oby.2008.607 [doi]
PST - ppublish
SO  - Obesity (Silver Spring). 2009 Apr;17(4):648-56. doi: 10.1038/oby.2008.607. Epub 
      2009 Jan 15.