PMID- 19149182
OWN - NLM
STAT- MEDLINE
DCOM- 20100126
LR  - 20090119
IS  - 1000-3061 (Print)
IS  - 1000-3061 (Linking)
VI  - 24
IP  - 10
DP  - 2008 Oct
TI  - [Immune responses induced by the suicidal DNA vaccines co-expressing the GP5 
      protein of PRRSV and the E2 protein of CSFV in mice].
PG  - 1714-22
AB  - Six recombinant plasmids co-expressing the wild-type GP5 gene or the 
      codon-optimized GP5 gene (containing pan-DR epitope) of porcine reproductive and 
      respiratory syndrome virus (PRRSV) and the E2 gene of classical swine fever virus 
      (CSFV) or the E2 fused with the UL49 of pseudorabies virus (PrV) were constructed 
      based on the suicidal DNA vaccine pSFV1CS-E2 described previously. Expression of 
      GP5 and E2 was confirmed by indirect immunofluorescence assay. The immunogenicity 
      of six plasmids was evaluated in BALB/c mouse model. For the six plasmids, 
      low-level of E2 and GP5 protein specific antibodies could be detected in the sera 
      of the immunized mice. Specific lymphoproliferative responses to the PRRSV or 
      CSFV stimulation were induced in the splenocytes of the immunized mice as 
      demonstrated by CFSE staining assay and WST-8 assay. Antigen specific IFN-gamma 
      and L-4 secretion was detected in the splenocytes of some immunized mice by 
      cytokine ELSIA. Fusion with the PrV UL49 in the suicidal vaccines induced 
      significantly higher lymphoproliferative responses and cytokine secretion. Taken 
      together, the suicidal DNA vaccines co-expressing GP5 and E2 could induce PRRSV 
      and CSFV specific humoral and cell-mediated immune responses.
FAU - Sun, Jianfu
AU  - Sun J
AD  - Division of Swine Infectious Diseases, National Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy 
      ofAgricultural Sciences, Harbin 150001, China.
FAU - Zhao, Heping
AU  - Zhao H
FAU - Li, Na
AU  - Li N
FAU - Sun, Yuan
AU  - Sun Y
FAU - Xi, Zhaohe
AU  - Xi Z
FAU - Zhou, Yanjun
AU  - Zhou Y
FAU - Wang, Yu
AU  - Wang Y
FAU - Qi, Qiaofen
AU  - Qi Q
FAU - Lu, Cheng
AU  - Lu C
FAU - Qiu, Huaji
AU  - Qiu H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - 0 (Antibodies, Viral)
RN  - 0 (Cytokines)
RN  - 0 (VP22 protein, Pseudorabies virus)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Structural Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
RN  - 0 (glycoprotein E2, classical swine fever virus)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Antibody Formation
MH  - Cytokines/blood
MH  - Female
MH  - Immunity, Cellular
MH  - Lymphocytes/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Random Allocation
MH  - Vaccines, DNA/biosynthesis/immunology
MH  - Viral Envelope Proteins/genetics/*immunology
MH  - Viral Structural Proteins/genetics/immunology
MH  - Viral Vaccines/biosynthesis/*immunology
EDAT- 2009/01/20 09:00
MHDA- 2010/01/27 06:00
CRDT- 2009/01/20 09:00
PHST- 2009/01/20 09:00 [entrez]
PHST- 2009/01/20 09:00 [pubmed]
PHST- 2010/01/27 06:00 [medline]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2008 Oct;24(10):1714-22.