PMID- 19150437
OWN - NLM
STAT- MEDLINE
DCOM- 20090421
LR  - 20141120
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1258
DP  - 2009 Mar 3
TI  - Brain-derived neurotrophic factor inhibits spontaneous inhibitory postsynaptic 
      currents in the rat supraoptic nucleus.
PG  - 34-42
LID - 10.1016/j.brainres.2008.12.057 [doi]
AB  - Body fluid balance requires the release of arginine vasopressin (AVP) from the 
      neurohypophysis. The hypothalamic supraoptic nucleus (SON) is one of the major 
      sites for the synthesis of AVP, and secretion of AVP is controlled by the 
      electrical activities of magnocellular neurosecretory cells (MNCs), which in turn 
      are regulated by neuronal excitatory glutamatergic and inhibitory GABAergic 
      inputs and humoral factors such as plasma osmolality. Previous studies have shown 
      that brain-derived neurotrophic factor (BDNF) mRNA was increased by osmotic 
      stress in the rat SON. In the present study, the effects of BDNF on excitatory 
      and inhibitory synaptic inputs were examined in the MNCs of rat SON, using the 
      whole-cell patch-clamp technique in in vitro brain slice preparations. BDNF 
      application caused a significant reduction in the frequency and amplitude of the 
      spontaneous inhibitory postsynaptic currents of the MNCs without affecting the 
      spontaneous excitatory postsynaptic currents. Next, whole-cell patch-clamp 
      recordings from dissociated SON MNCs expressing AVP-enhanced green fluorescent 
      protein (eGFP) transgene revealed that the amplitude of GABA-induced currents 
      were significantly smaller after BDNF treatment. Moreover, multi-cell reverse 
      transcriptase-polymerase chain reaction (RT-PCR) experiments revealed the 
      expression of TrkB mRNA in AVP-eGFP neurons. These results suggest that BDNF in 
      the rat SON may decrease the postsynaptic GABAergic activity and may be involved 
      in the regulatory mechanisms of body fluid homeostasis.
FAU - Ohbuchi, Toyoaki
AU  - Ohbuchi T
AD  - Department of Physiology, School of Medicine, University of Occupational and 
      Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
FAU - Yokoyama, Toru
AU  - Yokoyama T
FAU - Saito, Takeshi
AU  - Saito T
FAU - Hashimoto, Hirofumi
AU  - Hashimoto H
FAU - Suzuki, Hitoshi
AU  - Suzuki H
FAU - Otsubo, Hiroki
AU  - Otsubo H
FAU - Fujihara, Hiroaki
AU  - Fujihara H
FAU - Suzuki, Hideaki
AU  - Suzuki H
FAU - Ueta, Yoichi
AU  - Ueta Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081231
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carbazoles)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Indole Alkaloids)
RN  - 0 (RNA, Messenger)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - 97161-97-2 (staurosporine aglycone)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Carbazoles/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Excitatory Postsynaptic Potentials/physiology
MH  - Green Fluorescent Proteins/genetics
MH  - In Vitro Techniques
MH  - Indole Alkaloids/pharmacology
MH  - Inhibitory Postsynaptic Potentials/*physiology
MH  - Male
MH  - Neurons/*physiology
MH  - Neurosecretion
MH  - Patch-Clamp Techniques
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Transgenic
MH  - Rats, Wistar
MH  - Receptor, trkB/antagonists & inhibitors/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Supraoptic Nucleus/*physiology
MH  - gamma-Aminobutyric Acid/metabolism
EDAT- 2009/01/20 09:00
MHDA- 2009/04/22 09:00
CRDT- 2009/01/20 09:00
PHST- 2008/09/14 00:00 [received]
PHST- 2008/12/11 00:00 [revised]
PHST- 2008/12/17 00:00 [accepted]
PHST- 2009/01/20 09:00 [entrez]
PHST- 2009/01/20 09:00 [pubmed]
PHST- 2009/04/22 09:00 [medline]
AID - S0006-8993(08)03092-8 [pii]
AID - 10.1016/j.brainres.2008.12.057 [doi]
PST - ppublish
SO  - Brain Res. 2009 Mar 3;1258:34-42. doi: 10.1016/j.brainres.2008.12.057. Epub 2008 
      Dec 31.