PMID- 19150487
OWN - NLM
STAT- MEDLINE
DCOM- 20091019
LR  - 20211020
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 162
IP  - 3
DP  - 2009 Sep 1
TI  - Embryonic origins of ZebrinII parasagittal stripes and establishment of 
      topographic Purkinje cell projections.
PG  - 574-88
LID - 10.1016/j.neuroscience.2008.12.025 [doi]
AB  - The establishment of neural circuits involves both the precise positioning of 
      cells within brain regions and projection of axons to specific target cells. In 
      the cerebellum (Cb), the medial-lateral (M-L) and anterior-posterior (A-P) 
      position of each Purkinje cell (PC) and the topography of its axon can be defined 
      with respect to two coordinate systems within the Cb; one based on the pattern of 
      lobules and the other on PC gene expression in parasagittal clusters in the 
      embryo (e.g. Pcp2) and stripes in the adult (e.g. ZebrinII). The relationship 
      between the embryonic clusters of molecularly defined PCs and particular adult PC 
      stripes is not clear. Using a mouse genetic inducible fate mapping (GIFM) 
      approach and a Pcp2-CreER-IRES-hAP transgene, we marked three bilateral clusters 
      of PC clusters with myristolated green fluorescent protein (mGfp) on 
      approximately embryonic day (E) 15 and followed their fate into adulthood. We 
      found that these three clusters contributed specifically to ZebrinII-expressing 
      PCs, including nine of the adult stripes. This result suggests that embryonic PCs 
      maintain a particular molecular identity, and that each embryonic cluster can 
      contribute PCs to more than one adult M-L stripe. Each PC projects a primary axon 
      to one of the deep cerebellar nuclei (DCN) or the vestibular nuclei in the 
      brainstem in an organized fashion that relates to the position of the PCs along 
      the M-L axis. We characterized when PC axons from the three M-L clusters acquire 
      topographic projections. Using a combination of GIFM to mark the PC clusters with 
      mGfp and staining for human placental alkaline phosphatase (hAP) in 
      Pcp2-CreER-IRES-hAP transgenic embryos we found that axons from each embryonic PC 
      cluster intermingled with neurons within particular DCN or projected out of the 
      Cb toward the vestibular nuclei by E14.5. These studies show that PC molecular 
      patterning, efferent circuitry, and DCN nucleogenesis occur simultaneously, 
      suggesting a link between these processes.
FAU - Sillitoe, R V
AU  - Sillitoe RV
AD  - Developmental Biology Program, Sloan-Kettering Institute, 1275 York Avenue, New 
      York, NY 10021, USA.
FAU - Gopal, N
AU  - Gopal N
FAU - Joyner, A L
AU  - Joyner AL
LA  - eng
GR  - R01 CA128158/CA/NCI NIH HHS/United States
GR  - AS1658/AS/Autism Speaks/United States
GR  - R01 HD035768/HD/NICHD NIH HHS/United States
GR  - R01 HD035768-10/HD/NICHD NIH HHS/United States
GR  - R01 MH085726/MH/NIMH NIH HHS/United States
GR  - R01 MH085726-01/MH/NIMH NIH HHS/United States
GR  - R01 CA128158-11/CA/NCI NIH HHS/United States
GR  - R01 HD035768-11/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20081224
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (Pcp2 protein, mouse)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (Tbr1 protein, mouse)
RN  - 0 (zebrin II)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - EC 3.1.4.11 (Phospholipase C beta)
SB  - IM
MH  - Alkaline Phosphatase/genetics
MH  - Animals
MH  - Animals, Newborn
MH  - Axons/metabolism
MH  - Body Patterning/genetics/*physiology
MH  - Brain Mapping
MH  - Cerebellar Nuclei/cytology
MH  - Cerebellum/*cytology/embryology
MH  - DNA-Binding Proteins/metabolism
MH  - Embryo, Mammalian
MH  - Functional Laterality
MH  - Gene Expression Regulation, Developmental/genetics/*physiology
MH  - Green Fluorescent Proteins/genetics
MH  - Guanine Nucleotide Exchange Factors/genetics/metabolism
MH  - Humans
MH  - Mice
MH  - Mice, Transgenic
MH  - Nerve Tissue Proteins/*metabolism
MH  - Neural Pathways/metabolism
MH  - Neuropeptides/genetics/metabolism
MH  - Phospholipase C beta/metabolism
MH  - Purkinje Cells/cytology/*physiology
MH  - T-Box Domain Proteins
PMC - PMC2716412
MID - NIHMS84789
EDAT- 2009/01/20 09:00
MHDA- 2009/10/20 06:00
PMCR- 2010/09/01
CRDT- 2009/01/20 09:00
PHST- 2008/10/31 00:00 [received]
PHST- 2008/12/15 00:00 [revised]
PHST- 2008/12/15 00:00 [accepted]
PHST- 2009/01/20 09:00 [entrez]
PHST- 2009/01/20 09:00 [pubmed]
PHST- 2009/10/20 06:00 [medline]
PHST- 2010/09/01 00:00 [pmc-release]
AID - S0306-4522(08)01796-X [pii]
AID - 10.1016/j.neuroscience.2008.12.025 [doi]
PST - ppublish
SO  - Neuroscience. 2009 Sep 1;162(3):574-88. doi: 10.1016/j.neuroscience.2008.12.025. 
      Epub 2008 Dec 24.