PMID- 19151321
OWN - NLM
STAT- MEDLINE
DCOM- 20090928
LR  - 20211020
IS  - 1535-4989 (Electronic)
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 41
IP  - 3
DP  - 2009 Sep
TI  - Differential effects of dendritic cell transfer on airway hyperresponsiveness and 
      inflammation.
PG  - 271-80
LID - 10.1165/rcmb.2008-0256OC [doi]
AB  - Dendritic cells (DCs) are considered to be the most efficient antigen-presenting 
      cells. Intratracheal administration of allergen-pulsed bone marrow-derived 
      dendritic cells (BMDCs) before allergen challenge induces airway 
      hyperresponsiveness (AHR) and inflammation. Ovalbumin (OVA)-pulsed BMDCs from 
      wild-type (WT) mice were transferred into naive WT, CD4(-/-), CD8(-/-), or 
      IL-13(-/-) mice. Two days (short protocol) or 10 days (long protocol) after BMDC 
      transfer, mice were challenged with 1% OVA for 3 days and assayed 2 days later. 
      Transfer of OVA-primed BMDCs into BALB/c or C57BL/6 mice elicited AHR in both 
      protocols. Airway eosinophilia, Th2 cytokines, or goblet cell metaplasia were 
      increased in the long but not short protocol. Lung T cells from both protocols 
      produced Th2 cytokines in response to OVA in vitro. Carboxyfluorescein diacetate 
      succinimidylester-labeled BMDCs were observed in bronchoalveolar lavage (BAL) 
      fluid and lung parenchyma at early time points, and were detected in draining 
      lymph nodes 48 hours after transfer. CD8(-/-) mice developed AHR comparable to WT 
      mice in the short protocol, but decreased levels of AHR, airway eosinophilia, Th2 
      cytokines in BAL fluid, and goblet cell metaplasia compared with WT mice in the 
      long protocol. CD4(-/-) or IL-13(-/-) mice did not develop AHR or airway 
      inflammation in either protocol. These data suggest that allergen-pulsed BMDCs 
      initiate development of AHR that is dependent initially on CD4(+) T cells, and at 
      later time periods on CD8+ T cells and IL-13. Thus, the timing of allergen 
      challenge after transfer of allergen-pulsed BMDC affects the development of AHR 
      and airway inflammation.
FAU - Koya, Toshiyuki
AU  - Koya T
AD  - Department of Pediatrics, Division of Cell Biology, National Jewish Health, 1400 
      Jackson Street, Denver, CO 80206, USA.
FAU - Matsuda, Hiroyuki
AU  - Matsuda H
FAU - Matsubara, Shigeki
AU  - Matsubara S
FAU - Miyahara, Nobuaki
AU  - Miyahara N
FAU - Dakhama, Azzeddine
AU  - Dakhama A
FAU - Takeda, Katsuyuki
AU  - Takeda K
FAU - Gelfand, Erwin W
AU  - Gelfand EW
LA  - eng
GR  - AI-77609/AI/NIAID NIH HHS/United States
GR  - HL-36577/HL/NHLBI NIH HHS/United States
GR  - HL-61005/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090116
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (CD4 Antigens)
RN  - 0 (CD8 Antigens)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-13)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Bronchial Hyperreactivity/*immunology/pathology
MH  - Bronchoalveolar Lavage Fluid/immunology
MH  - CD4 Antigens/genetics/immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8 Antigens/genetics/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cytokines/immunology
MH  - Dendritic Cells/cytology/*immunology
MH  - Female
MH  - Goblet Cells/immunology/pathology
MH  - Inflammation/*immunology
MH  - Interleukin-13/genetics/immunology
MH  - Lymphocyte Activation/immunology
MH  - Lymphocyte Subsets
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Ovalbumin/immunology
PMC - PMC2742748
EDAT- 2009/01/20 09:00
MHDA- 2009/09/29 06:00
PMCR- 2010/09/01
CRDT- 2009/01/20 09:00
PHST- 2009/01/20 09:00 [entrez]
PHST- 2009/01/20 09:00 [pubmed]
PHST- 2009/09/29 06:00 [medline]
PHST- 2010/09/01 00:00 [pmc-release]
AID - 2008-0256OC [pii]
AID - ajrcmb413271 [pii]
AID - 10.1165/rcmb.2008-0256OC [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 2009 Sep;41(3):271-80. doi: 10.1165/rcmb.2008-0256OC. 
      Epub 2009 Jan 16.