PMID- 19151393
OWN - NLM
STAT- MEDLINE
DCOM- 20090611
LR  - 20211020
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 50
IP  - 6
DP  - 2009 Jun
TI  - Heat-induced reactivation of HSV-1 in latent mice: upregulation in the TG of CD83 
      and other immune response genes and their LAT-ICP0 locus.
PG  - 2855-61
LID - 10.1167/iovs.08-2430 [doi]
AB  - PURPOSE: To determine changes in host gene expression in HSV-1 latent trigeminal 
      ganglia (TG) after hyperthermic stress. METHODS: Scarified corneas of 6-week-old 
      female BALB/c mice were inoculated with either HSV-1 17Syn(+) (high phenotypic 
      reactivator) or 17DeltaPst(LAT(-)) (low phenotypic reactivator) at 10(4) 
      plaque-forming units/eye. At 28 days after infection, viral reactivation was 
      induced in some of the infected mice with hyperthermic stress, and the mice were 
      killed after 1 hour. Heat-treated uninfected mice served as the control. Labeled 
      cRNA derived from TG-isolated total RNA was hybridized to 430 2.0 chips 
      containing 14,000 mouse genes. Gene expression was confirmed by quantitative 
      real-time PCR. RESULTS: There was no difference in gene expression in the 
      non-heat-treated mice. Gene expression in the TG of each of the heat-treated 
      mouse groups (17Syn(+), 17DeltaPst(LAT(-)) and uninfected) yielded upregulation 
      of more than twofold of a group of the same genes, designated as heat 
      stress-induced gene expression. Twenty-nine genes (0.2%) were significantly 
      upregulated (2- to 17-fold) when the heat stress-induced gene expression was 
      subtracted from the gene expression of 17Syn(+) latent TG relative to 
      17DeltaPst(LAT(-)) latent TG 1 hour after mouse hyperthermic stress. Nine host 
      adaptive immunity genes comprising Ig molecules, CD83, CD8A, ADA, and CCL8 were 
      the largest subset upregulated, and all were confirmed by real-time PCR. Others 
      identified included genes involved in hypothalamic-pituitary gland functions. 
      CONCLUSIONS: Hyperthermic stress-induced reactivation of the HSV-1 high 
      phenotypic reactivator can upregulate gene expression involved in B-cell function 
      and in T-cell function. CD83 is implicated in HSV-1 latency, suggesting it could 
      also be involved in immune-mediated mechanisms of viral reactivation.
FAU - Clement, Christian
AU  - Clement C
AD  - Department of Ophthalmology, LSU Eye Center, Louisiana State University Health 
      Sciences Center, New Orleans, Louisiana 70112, USA.
FAU - Bhattacharjee, Partha S
AU  - Bhattacharjee PS
FAU - Kaufman, Herbert E
AU  - Kaufman HE
FAU - Hill, James M
AU  - Hill JM
LA  - eng
GR  - R01 EY006311/EY/NEI NIH HHS/United States
GR  - R01 EY002672-28/EY/NEI NIH HHS/United States
GR  - P30 EY002377-259001/EY/NEI NIH HHS/United States
GR  - R01 EY006311-21/EY/NEI NIH HHS/United States
GR  - P30 EY002377-309006/EY/NEI NIH HHS/United States
GR  - R01 EY006311-20A1/EY/NEI NIH HHS/United States
GR  - P30 EY002377-299006/EY/NEI NIH HHS/United States
GR  - P30 EY002377-279006/EY/NEI NIH HHS/United States
GR  - EY002622/EY/NEI NIH HHS/United States
GR  - R01 EY002672-27A1/EY/NEI NIH HHS/United States
GR  - P30 EY002377-289006/EY/NEI NIH HHS/United States
GR  - R01 EY006311-19S1/EY/NEI NIH HHS/United States
GR  - P30 EY002377-209001/EY/NEI NIH HHS/United States
GR  - P30 EY002377-269006/EY/NEI NIH HHS/United States
GR  - P30 EY002377-239001/EY/NEI NIH HHS/United States
GR  - P30 EY002377-219001/EY/NEI NIH HHS/United States
GR  - EY02377/EY/NEI NIH HHS/United States
GR  - P30 EY002377/EY/NEI NIH HHS/United States
GR  - P30 EY002377-229001/EY/NEI NIH HHS/United States
GR  - R01 EY006311-23/EY/NEI NIH HHS/United States
GR  - R01 EY002672-29/EY/NEI NIH HHS/United States
GR  - R01 EY006311-22/EY/NEI NIH HHS/United States
GR  - P30 EY002377-199001/EY/NEI NIH HHS/United States
GR  - R01 EY002672-30/EY/NEI NIH HHS/United States
GR  - R01 EY006311-22S1/EY/NEI NIH HHS/United States
GR  - EY006311/EY/NEI NIH HHS/United States
GR  - P30 EY002377-249001/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090117
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (CD8 Antigens)
RN  - 0 (CD8 antigen, alpha chain)
RN  - 0 (Ccl8 protein, mouse)
RN  - 0 (Chemokine CCL8)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (MicroRNAs)
RN  - 0 (latency associated transcript, herpes simplex virus-1)
RN  - EC 3.2.2.5 (Cd38 protein, mouse)
RN  - EC 3.2.2.6 (ADP-ribosyl Cyclase 1)
SB  - IM
MH  - ADP-ribosyl Cyclase 1/*genetics
MH  - Animals
MH  - CD8 Antigens/genetics
MH  - Chemokine CCL8/genetics
MH  - Cornea/*innervation/virology
MH  - Female
MH  - Gene Expression Regulation/*physiology
MH  - Genes, MHC Class II/*genetics
MH  - Herpesvirus 1, Human/*physiology
MH  - Hot Temperature
MH  - Membrane Glycoproteins/*genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/*genetics
MH  - Oligonucleotide Array Sequence Analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stress, Physiological
MH  - Trigeminal Ganglion/*virology
MH  - Virus Activation
PMC - PMC2702237
MID - NIHMS122439
EDAT- 2009/01/20 09:00
MHDA- 2009/06/12 09:00
PMCR- 2010/06/01
CRDT- 2009/01/20 09:00
PHST- 2009/01/20 09:00 [entrez]
PHST- 2009/01/20 09:00 [pubmed]
PHST- 2009/06/12 09:00 [medline]
PHST- 2010/06/01 00:00 [pmc-release]
AID - iovs.08-2430 [pii]
AID - 10.1167/iovs.08-2430 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2009 Jun;50(6):2855-61. doi: 10.1167/iovs.08-2430. 
      Epub 2009 Jan 17.