PMID- 19152859
OWN - NLM
STAT- MEDLINE
DCOM- 20090302
LR  - 20090120
IS  - 1557-7988 (Electronic)
IS  - 0076-6879 (Linking)
VI  - 450
DP  - 2008
TI  - Application of single-molecule spectroscopy in studying enzyme kinetics and 
      mechanism.
PG  - 129-57
LID - 10.1016/S0076-6879(08)03407-1 [doi]
AB  - This chapter reviews recent developments in the application of single-molecule 
      spectroscopy (SMS) to studies of enzyme kinetics and mechanism. Protocols for 
      conducting single-molecule experiments on enzymes, based largely on the 
      experience in our laboratory, are provided, including methods of sample 
      preparation, instrumentation, and data analysis. We also address general issues 
      related to the design of meaningful single-molecule experiments and include 
      specific examples of the application of SMS to enzyme studies, which reveal new 
      and intriguing aspects of enzyme behavior, including static and dynamic 
      heterogeneity, as well as subunit cooperativity. Finally, we discuss the 
      advantages of employing single-molecule approach in obtaining new information 
      beyond ensemble studies.
FAU - Shi, Jue
AU  - Shi J
AD  - Biophysics Research Division, University of Michigan, Ann Arbor, Michigan, USA.
FAU - Dertouzos, Joseph
AU  - Dertouzos J
FAU - Gafni, Ari
AU  - Gafni A
FAU - Steel, Duncan
AU  - Steel D
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
RN  - 0 (Enzymes)
SB  - IM
MH  - Enzymes/chemistry/*metabolism
MH  - Kinetics
MH  - Spectrometry, Fluorescence/*methods
RF  - 48
EDAT- 2009/01/21 09:00
MHDA- 2009/03/03 09:00
CRDT- 2009/01/21 09:00
PHST- 2009/01/21 09:00 [entrez]
PHST- 2009/01/21 09:00 [pubmed]
PHST- 2009/03/03 09:00 [medline]
AID - S0076-6879(08)03407-1 [pii]
AID - 10.1016/S0076-6879(08)03407-1 [doi]
PST - ppublish
SO  - Methods Enzymol. 2008;450:129-57. doi: 10.1016/S0076-6879(08)03407-1.