PMID- 19153640
OWN - NLM
STAT- MEDLINE
DCOM- 20090408
LR  - 20090213
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 154
IP  - 2
DP  - 2009
TI  - Relationship between U83 gene variation in human herpesvirus 6 and secretion of 
      the U83 gene product.
PG  - 273-83
LID - 10.1007/s00705-008-0307-3 [doi]
AB  - The betaherpesvirus human herpesvirus 6 (HHV-6) has two variants. The U83 gene 
      product of strain HST is a chemoattractant for monocytes. Here, we describe U83 
      gene variations that accumulated in variants A and B. A gene-variation hot spot 
      was examined in 36 different strains and one donor DNA sample. U83 gene 
      variations accumulated in variant A and in reactivated variant B after 
      transplantation. None of the variant-A viruses encoded the signal peptide found 
      in the B variant. U83 gene sequencing suggested that the variant A and B groups 
      were separate, and that the variant B viruses could be further divided into the 
      HST-Z29 type and another type with a shorter signal peptide. In a eukaryotic 
      expression system, the HST-Z29 type of U83 gene product was secreted into the 
      medium, a frame-shifted HST-Z29 type was partially secreted, and the variant-A 
      type and a first-methionine knockout of the HST-Z29 type were not secreted.
FAU - Sjahril, R
AU  - Sjahril R
AD  - Department of Infectious Disease Control, Graduate School of Medicine G5, Osaka 
      University, 2-2 Yamada-Oka Suita, Osaka 565-0871, Japan.
FAU - Isegawa, Yuji
AU  - Isegawa Y
FAU - Tanaka, T
AU  - Tanaka T
FAU - Nakano, K
AU  - Nakano K
FAU - Yoshikawa, T
AU  - Yoshikawa T
FAU - Asano, Y
AU  - Asano Y
FAU - Ohshima, A
AU  - Ohshima A
FAU - Yamanishi, K
AU  - Yamanishi K
FAU - Sugimoto, N
AU  - Sugimoto N
LA  - eng
PT  - Journal Article
DEP - 20090120
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Chemokines)
RN  - 0 (U83 protein, Human herpesvirus 6)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Chemokines/genetics/*metabolism
MH  - Cloning, Molecular
MH  - Democratic Republic of the Congo
MH  - Gene Frequency
MH  - Genetic Variation
MH  - Germany
MH  - Herpesvirus 6, Human/genetics/isolation & purification/*metabolism
MH  - Humans
MH  - Japan
MH  - Molecular Sequence Data
MH  - Roseolovirus Infections/*metabolism/virology
MH  - Sequence Alignment
MH  - Sequence Homology
MH  - United States
MH  - Viral Proteins/genetics/*metabolism
EDAT- 2009/01/21 09:00
MHDA- 2009/04/09 09:00
CRDT- 2009/01/21 09:00
PHST- 2008/10/31 00:00 [received]
PHST- 2008/12/09 00:00 [accepted]
PHST- 2009/01/21 09:00 [entrez]
PHST- 2009/01/21 09:00 [pubmed]
PHST- 2009/04/09 09:00 [medline]
AID - 10.1007/s00705-008-0307-3 [doi]
PST - ppublish
SO  - Arch Virol. 2009;154(2):273-83. doi: 10.1007/s00705-008-0307-3. Epub 2009 Jan 20.