PMID- 19154183
OWN - NLM
STAT- MEDLINE
DCOM- 20090414
LR  - 20161125
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 419
IP  - 2
DP  - 2009 Apr 15
TI  - Role of aryl hydrocarbon receptor in modulation of the expression of the hypoxia 
      marker carbonic anhydrase IX.
PG  - 419-25
LID - 10.1042/BJ20080952 [doi]
AB  - Tumour-associated expression of CA IX (carbonic anhydrase IX) is to a major 
      extent regulated by HIF-1 (hypoxia-inducible factor-1) which is important for 
      transcriptional activation and consists of the oxygen-regulated subunit 
      HIF-1alpha and the partner factor ARNT [AhR (aryl hydrocarbon receptor) nuclear 
      translocator]. We have previously observed that HIF-1alpha competes with the AhR 
      for interaction with ARNT under conditions when both conditionally regulated 
      factors are activated. We have therefore investigated whether TCDD 
      (2,3,7,8-tetrachlorodibenzo-p-dioxin)-induced activation of the AhR pathway might 
      interfere with CA IX expression. The results from the present study suggest that 
      TCDD treatment reduces hypoxic induction of both CA IX mRNA and protein 
      expression. Moreover, the transcriptional activity of the CA9 promoter was 
      significantly reduced by expression of CAAhR (constitutively active AhR), which 
      activates transcription in a ligand-independent manner. Finally, we found that 
      ARNT is critical for both hypoxic induction and the TCDD-mediated inhibition of 
      CA9 expression.
FAU - Takacova, Martina
AU  - Takacova M
AD  - Institute of Virology, Centre of Molecular Medicine, Slovak Academy of Sciences, 
      Dubravska cesta 9, 845 05 Bratislava, Slovak Republic.
FAU - Holotnakova, Tereza
AU  - Holotnakova T
FAU - Vondracek, Jan
AU  - Vondracek J
FAU - Machala, Miroslav
AU  - Machala M
FAU - Pencikova, Katerina
AU  - Pencikova K
FAU - Gradin, Katarina
AU  - Gradin K
FAU - Poellinger, Lorenz
AU  - Poellinger L
FAU - Pastorek, Jaromir
AU  - Pastorek J
FAU - Pastorekova, Silvia
AU  - Pastorekova S
FAU - Kopacek, Juraj
AU  - Kopacek J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - EC 4.2.1.1 (CA9 protein, human)
RN  - EC 4.2.1.1 (Carbonic Anhydrase IX)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
SB  - IM
MH  - Animals
MH  - Antigens, Neoplasm/genetics
MH  - Binding Sites
MH  - Blotting, Western
MH  - Carbonic Anhydrase IX
MH  - Carbonic Anhydrases/genetics/*metabolism
MH  - Cell Hypoxia/genetics/*physiology
MH  - Cell Line, Tumor
MH  - Chromatin Immunoprecipitation
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Mice
MH  - Polychlorinated Dibenzodioxins/pharmacology
MH  - Polymerase Chain Reaction
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Binding/drug effects
MH  - Receptors, Aryl Hydrocarbon/genetics/metabolism/*physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/drug effects
EDAT- 2009/01/22 09:00
MHDA- 2009/04/15 09:00
CRDT- 2009/01/22 09:00
PHST- 2009/01/22 09:00 [entrez]
PHST- 2009/01/22 09:00 [pubmed]
PHST- 2009/04/15 09:00 [medline]
AID - BJ20080952 [pii]
AID - 10.1042/BJ20080952 [doi]
PST - ppublish
SO  - Biochem J. 2009 Apr 15;419(2):419-25. doi: 10.1042/BJ20080952.