PMID- 19154347
OWN - NLM
STAT- MEDLINE
DCOM- 20090327
LR  - 20090213
IS  - 1742-4658 (Electronic)
IS  - 1742-464X (Linking)
VI  - 276
IP  - 4
DP  - 2009 Feb
TI  - Transcription factor specificity protein 1 (SP1) and activating protein 2alpha 
      (AP-2alpha) regulate expression of human KCTD10 gene by binding to proximal 
      region of promoter.
PG  - 1114-24
LID - 10.1111/j.1742-4658.2008.06855.x [doi]
AB  - Potassium channel tetramerization domain-containing 10 gene (KCTD10) belongs to 
      the polymerase delta-interacting protein 1 (PDIP1) gene family. Mouse KCTD10 was 
      thought to interact with proliferating cell nuclear antigen and the small subunit 
      of polymerase delta, and to have roles in DNA repair, DNA replication and 
      cell-cycle control. To better understand the regulatory mechanism of KCTD10 
      expression, we characterized the promoter of human KCTD10 containing a 639 bp 
      fragment of the 5'-flanking region (-609/+30). A primer extension assay 
      identified the transcription start site as an A, 63 bp upstream of the start 
      codon. The promoter region contains neither a TATA box nor a CCAAT box, but a CpG 
      island was found near to the transcription start site. Deletion mutagenesis 
      showed that the region from -108 to +30 was indispensable to the promoter 
      activity, and site-directed mutation analysis in this proximal promoter region of 
      KCTD10 revealed two important transcription regulatory elements of specificity 
      protein 1 (SP1) and activating protein-2 (AP-2). An in vivo chromatin 
      immunoprecipitation assay further demonstrated that SP1 and AP-2alpha could bind 
      to this proximal promoter region. Moreover, using a luciferase reporter assay, 
      quantitative real-time RT-PCR and western blot analysis, both overexpression and 
      RNA interference of SP1 and AP-2alpha indicated that the binding of SP1 to the 
      proximal promoter region stimulated the promoter activity and endogenous KCTD10 
      expression, whereas binding of AP-2alpha to this region showed opposite effects.
FAU - Liu, Rushi
AU  - Liu R
AD  - Key Laboratory of Protein Biochemistry and Development Biology of State Education 
      Ministry of China, Hunan Normal University, China.
FAU - Zhou, Aidong
AU  - Zhou A
FAU - Ren, Daolong
AU  - Ren D
FAU - He, Ailan
AU  - He A
FAU - Hu, Xiang
AU  - Hu X
FAU - Zhang, Wenfeng
AU  - Zhang W
FAU - Yang, Liping
AU  - Yang L
FAU - Liu, Mingjun
AU  - Liu M
FAU - Li, Hong
AU  - Li H
FAU - Zhou, Jianlin
AU  - Zhou J
FAU - Xiang, Shuanglin
AU  - Xiang S
FAU - Zhang, Jian
AU  - Zhang J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090116
PL  - England
TA  - FEBS J
JT  - The FEBS journal
JID - 101229646
RN  - 0 (KCTD10 protein, human)
RN  - 0 (Potassium Channels, Voltage-Gated)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (Transcription Factor AP-2)
SB  - IM
MH  - Base Sequence
MH  - Binding Sites
MH  - Cell Line
MH  - CpG Islands
MH  - Gene Expression Regulation
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Potassium Channels, Voltage-Gated/*biosynthesis
MH  - Promoter Regions, Genetic
MH  - Sp1 Transcription Factor/*physiology
MH  - Transcription Factor AP-2/*physiology
EDAT- 2009/01/22 09:00
MHDA- 2009/03/28 09:00
CRDT- 2009/01/22 09:00
PHST- 2009/01/22 09:00 [entrez]
PHST- 2009/01/22 09:00 [pubmed]
PHST- 2009/03/28 09:00 [medline]
AID - EJB6855 [pii]
AID - 10.1111/j.1742-4658.2008.06855.x [doi]
PST - ppublish
SO  - FEBS J. 2009 Feb;276(4):1114-24. doi: 10.1111/j.1742-4658.2008.06855.x. Epub 2009 
      Jan 16.