PMID- 19155326
OWN - NLM
STAT- MEDLINE
DCOM- 20090306
LR  - 20211020
IS  - 1469-9001 (Electronic)
IS  - 1355-8382 (Print)
IS  - 1355-8382 (Linking)
VI  - 15
IP  - 3
DP  - 2009 Mar
TI  - A dominant mutation in DCL1 suppresses the hyl1 mutant phenotype by promoting the 
      processing of miRNA.
PG  - 450-8
LID - 10.1261/rna.1297109 [doi]
AB  - MicroRNAs (miRNAs) are sequence-specific negative regulators of gene expression 
      generated by DICER-LIKE1 (DCL1) with the assistance of a double-stranded 
      RNA-binding protein, HYPONASTIC LEAVES1 (HYL1), in Arabidopsis. To achieve a 
      better understanding of miRNA biogenesis, we isolated hyl1 suppressors. Our 
      genetic screening identified a novel semidominant mutation in DCL1 (dcl1-13), 
      which causes an amino acid substitution of Glu-395 with Lys in the 
      ATPase/DExH-box RNA helicase domain. This mutation confers significant 
      restoration from the developmental abnormality and a reduction in the level of 
      miRNA in the loss-of-function mutant of HYL1. However, the dcl1-13 single mutant, 
      exhibiting a decreased number of leaves, showed a slight decrease in miRNA 
      accumulation. Thus, the effect of the dcl1-13 mutation is HYL1 dependent: it 
      promotes miRNA processing in the absence of HYL1, but conversely, impairs it in 
      the presence of HYL1. Our results suggest significant roles of the helicase 
      domain of DCL1, which remain unclear to date, possibly in relation with HYL1.
FAU - Tagami, Yuko
AU  - Tagami Y
AD  - Department of Life Sciences, Graduate School of Arts and Sciences, The University 
      of Tokyo, Meguro, Tokyo 153-8902, Japan.
FAU - Motose, Hiroyasu
AU  - Motose H
FAU - Watanabe, Yuichiro
AU  - Watanabe Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090120
PL  - United States
TA  - RNA
JT  - RNA (New York, N.Y.)
JID - 9509184
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (HYL1 protein, Arabidopsis)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Plant)
RN  - 0 (RNA-Binding Proteins)
RN  - EC 3.1.26.3 (DCL1 protein, Arabidopsis)
RN  - EC 3.1.26.3 (Ribonuclease III)
SB  - IM
MH  - Amino Acid Substitution
MH  - Arabidopsis/*genetics/*metabolism
MH  - Arabidopsis Proteins/chemistry/*genetics/metabolism
MH  - Cell Cycle Proteins/chemistry/*genetics/metabolism
MH  - MicroRNAs/metabolism
MH  - Models, Molecular
MH  - Mutation
MH  - Phenotype
MH  - RNA, Plant/metabolism
MH  - RNA-Binding Proteins/chemistry/*genetics/metabolism
MH  - Ribonuclease III/chemistry/*genetics/metabolism
PMC - PMC2657008
EDAT- 2009/01/22 09:00
MHDA- 2009/03/07 09:00
PMCR- 2009/09/01
CRDT- 2009/01/22 09:00
PHST- 2009/01/22 09:00 [entrez]
PHST- 2009/01/22 09:00 [pubmed]
PHST- 2009/03/07 09:00 [medline]
PHST- 2009/09/01 00:00 [pmc-release]
AID - rna.1297109 [pii]
AID - RA [pii]
AID - 10.1261/rna.1297109 [doi]
PST - ppublish
SO  - RNA. 2009 Mar;15(3):450-8. doi: 10.1261/rna.1297109. Epub 2009 Jan 20.