PMID- 19155473
OWN - NLM
STAT- MEDLINE
DCOM- 20090330
LR  - 20211020
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 182
IP  - 3
DP  - 2009 Feb 1
TI  - A new triggering receptor expressed on myeloid cells (Trem) family member, 
      Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linked 
      marker for subsets of mouse macrophages and dendritic cells.
PG  - 1278-86
AB  - Dendritic cells (DCs) are professional APCs that can control immune responses 
      against self and altered self, typically foreign, determinants. DCs can be 
      divided into several subsets, including CD8alpha(+) and CD8alpha(-) DCs. These 
      subsets possess specific functions. For example, mouse splenic CD8alpha(+), but 
      not CD8alpha(-) DCs selectively take up dying cells and cross-present 
      cell-associated Ags to naive T cells. In this study, we identified genes that 
      were more expressed in CD8alpha(+) than CD8alpha(-) DCs by microarray analysis. 
      Only one of these genes, when the extracellular domains were linked to human IgG 
      Fc domain, could bind to late apoptotic or necrotic cells. This gene was a new 
      member of the triggering receptor expressed on myeloid cells (Trem) family, 
      Trem-like 4 (Treml4). Treml4 mRNA and protein, the latter detected with a new 
      mAb, were predominantly expressed in spleen. Treml4, like other Trem family 
      members, could associate with the adaptor molecule DNAX activation protein 12 
      kDa, but neither DNAX activation protein 10 kDa nor FcRgamma. Consistent with the 
      microarray data, we confirmed that Treml4 protein was more expressed on 
      CD8alpha(+) than CD8alpha(-) DCs, and we also found that Treml4 was expressed at 
      high levels on splenic macrophages in spleen, particularly red pulp and marginal 
      metallophilic macrophages. In addition, Treml4 expression on DCs was not changed 
      after maturation induced by TLR ligands. Thus, Treml4 is a new Trem family 
      molecule that is abundantly expressed on CD8alpha(+) DCs and subsets of splenic 
      resident macrophages, and can recognize dead cells by different types of 
      phagocytes in spleen.
FAU - Hemmi, Hiroaki
AU  - Hemmi H
AD  - Medical Top Track Program, Medical Research Institute, Tokyo Medical and Dental 
      University, Tokyo, Japan. hemmi.mtt@mri.tmd.ac.jp
FAU - Idoyaga, Juliana
AU  - Idoyaga J
FAU - Suda, Koji
AU  - Suda K
FAU - Suda, Nao
AU  - Suda N
FAU - Kennedy, Kathleen
AU  - Kennedy K
FAU - Noda, Masaki
AU  - Noda M
FAU - Aderem, Alan
AU  - Aderem A
FAU - Steinman, Ralph M
AU  - Steinman RM
LA  - eng
SI  - GEO/GSE13250
GR  - R01 AI013013-31/AI/NIAID NIH HHS/United States
GR  - R01 AI013013-26/AI/NIAID NIH HHS/United States
GR  - R01 AI013013-29/AI/NIAID NIH HHS/United States
GR  - R01 AI013013-32/AI/NIAID NIH HHS/United States
GR  - R01 AI013013-24/AI/NIAID NIH HHS/United States
GR  - R01 AI013013-25/AI/NIAID NIH HHS/United States
GR  - AI13013/AI/NIAID NIH HHS/United States
GR  - R01 AI013013-28/AI/NIAID NIH HHS/United States
GR  - R01 AI013013-23/AI/NIAID NIH HHS/United States
GR  - R01 AI013013-33/AI/NIAID NIH HHS/United States
GR  - R01 AI013013-30/AI/NIAID NIH HHS/United States
GR  - R01 AI013013/AI/NIAID NIH HHS/United States
GR  - R01 AI013013-27/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Biomarkers)
RN  - 0 (CD8 Antigens)
RN  - 0 (CD8 antigen, alpha chain)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Natural Killer Cell)
RN  - 0 (Treml4 protein, mouse)
RN  - 0 (Tyrobp protein, mouse)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/*metabolism
MH  - Animals
MH  - Biomarkers/metabolism
MH  - CD8 Antigens/biosynthesis/metabolism
MH  - Cell Adhesion/immunology
MH  - Cell Death/immunology
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Dendritic Cells/chemistry/cytology/*metabolism
MH  - Humans
MH  - Macrophages/chemistry/cytology/*metabolism
MH  - Melanoma, Experimental
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Myeloid Cells/chemistry/cytology/*metabolism
MH  - Phagocytes/cytology/immunology/metabolism
MH  - Protein Binding/immunology
MH  - Receptors, Immunologic/*metabolism
MH  - Receptors, Natural Killer Cell/metabolism
MH  - Spleen/cytology/immunology/metabolism
PMC - PMC2843158
MID - NIHMS107081
COIS- Disclosure The authors have no financial conflict of interest.
EDAT- 2009/01/22 09:00
MHDA- 2009/03/31 09:00
PMCR- 2010/03/22
CRDT- 2009/01/22 09:00
PHST- 2009/01/22 09:00 [entrez]
PHST- 2009/01/22 09:00 [pubmed]
PHST- 2009/03/31 09:00 [medline]
PHST- 2010/03/22 00:00 [pmc-release]
AID - 182/3/1278 [pii]
AID - 10.4049/jimmunol.182.3.1278 [doi]
PST - ppublish
SO  - J Immunol. 2009 Feb 1;182(3):1278-86. doi: 10.4049/jimmunol.182.3.1278.