PMID- 19156770
OWN - NLM
STAT- MEDLINE
DCOM- 20090720
LR  - 20180924
IS  - 1522-2683 (Electronic)
IS  - 0173-0835 (Linking)
VI  - 30
IP  - 4
DP  - 2009 Feb
TI  - A rapid MEKC method for the simultaneous determination of creatinine, 1- and 
      3-methylhistidine in human urine.
PG  - 654-6
LID - 10.1002/elps.200800565 [doi]
AB  - The present report describes the use of MEKC in the presence of 35 mM sodium 
      tetraborate, pH 9.0, containing 60 mM SDS for the complete separation and 
      identification of creatinine, 1-methylhistidine (1-MeH) and 3-MeH in human urine. 
      Their simultaneous quantification in urine of healthy controls and subjects 
      submitted to elective surgery to their lower limbs allowed to use 3-MeH as a 
      reliable measure of skeletal protein breakdown. Simplicity, sensitivity and low 
      running costs are the main advantages of this method that is suitable for the 
      routine analysis in clinical laboratories of a large number of samples per day.
FAU - Ferrari, Fabio
AU  - Ferrari F
AD  - Department of Biochemistry A. Castellani, University of Pavia, Pavia, Italy.
FAU - Fumagalli, Marco
AU  - Fumagalli M
FAU - Viglio, Simona
AU  - Viglio S
FAU - Aquilani, Roberto
AU  - Aquilani R
FAU - Pasini, Evasio
AU  - Pasini E
FAU - Iadarola, Paolo
AU  - Iadarola P
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Methylhistidines)
RN  - 0 (Muscle Proteins)
RN  - 332-80-9 (1-methylhistidine)
RN  - AYI8EX34EU (Creatinine)
RN  - MEH8O8Y0H0 (3-methylhistidine)
SB  - IM
MH  - Chromatography, Micellar Electrokinetic Capillary/*methods
MH  - Creatinine/*urine
MH  - Humans
MH  - Methylhistidines/*urine
MH  - Muscle Proteins/metabolism
MH  - Sensitivity and Specificity
EDAT- 2009/01/22 09:00
MHDA- 2009/07/21 09:00
CRDT- 2009/01/22 09:00
PHST- 2009/01/22 09:00 [entrez]
PHST- 2009/01/22 09:00 [pubmed]
PHST- 2009/07/21 09:00 [medline]
AID - 10.1002/elps.200800565 [doi]
PST - ppublish
SO  - Electrophoresis. 2009 Feb;30(4):654-6. doi: 10.1002/elps.200800565.