PMID- 19156792
OWN - NLM
STAT- MEDLINE
DCOM- 20090326
LR  - 20090219
IS  - 1521-3773 (Electronic)
IS  - 1433-7851 (Linking)
VI  - 48
IP  - 9
DP  - 2009
TI  - Proteomic analysis of plasma membrane vesicles.
PG  - 1656-9
LID - 10.1002/anie.200803898 [doi]
AB  - A simple and scalable method is presented for harvesting, purification, and 
      on-chip processing of mammalian plasma membrane vesicles (PMVs) optimized for 
      downstream proteome analysis. After immobilization on a microfluidic flowcell of 
      PMVs, the embedded membrane proteins are proteolytically digested, and the 
      peptides harvested and analyzed by LC-MS/MS. Over 93% of the detected proteins 
      are plasma-membrane-derived.
FAU - Bauer, Brigitte
AU  - Bauer B
AD  - Department of Chemical and Biological Engineering, Chalmers University of 
      Technology, 41296 Goteborg, Sweden.
FAU - Davidson, Max
AU  - Davidson M
FAU - Orwar, Owe
AU  - Orwar O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Angew Chem Int Ed Engl
JT  - Angewandte Chemie (International ed. in English)
JID - 0370543
RN  - 0 (Membrane Proteins)
RN  - 0 (Peptides)
SB  - IM
MH  - Cell Membrane/*chemistry
MH  - Chromatography, Liquid
MH  - Membrane Proteins/analysis/*chemistry/metabolism
MH  - Microfluidics/methods
MH  - Peptides/analysis
MH  - Proteomics/*methods
MH  - Tandem Mass Spectrometry
EDAT- 2009/01/22 09:00
MHDA- 2009/03/27 09:00
CRDT- 2009/01/22 09:00
PHST- 2009/01/22 09:00 [entrez]
PHST- 2009/01/22 09:00 [pubmed]
PHST- 2009/03/27 09:00 [medline]
AID - 10.1002/anie.200803898 [doi]
PST - ppublish
SO  - Angew Chem Int Ed Engl. 2009;48(9):1656-9. doi: 10.1002/anie.200803898.