PMID- 19157828
OWN - NLM
STAT- MEDLINE
DCOM- 20100201
LR  - 20111117
IS  - 1873-4847 (Electronic)
IS  - 0955-2863 (Linking)
VI  - 21
IP  - 1
DP  - 2010 Jan
TI  - Oligomerized grape seed polyphenols attenuate inflammatory changes due to 
      antioxidative properties in coculture of adipocytes and macrophages.
PG  - 47-54
LID - 10.1016/j.jnutbio.2008.10.003 [doi]
AB  - Macrophage infiltration of white adipose tissue (WAT) is implicated in the 
      metabolic complications of obesity. In addition, inflammatory changes through 
      dysregulated expression of inflammation-related adipokines such as tumor necrosis 
      factor-alpha (TNF-alpha) and monocyte chemoattractant protein-1 (MCP-1) in WAT 
      are considered to be one of the causes of insulin resistance. Recently, enhanced 
      oxidative stress in adipocytes has been reported to be implicated in dysregulated 
      expression of inflammation-related adipokines. Polyphenols are well known as 
      potent natural antioxidants in the diet. In the present study, we investigated 
      the antioxidative effects of an oligomerized grape seed polyphenol (OGSP) on 
      inflammatory changes in coculture of adipocytes and macrophages. Coculture of HW 
      mouse white adipocytes and RAW264 mouse macrophages markedly increased the 
      production of TNF-alpha, MCP-1 and plasminogen activator inhibitor-1 compared 
      with control culture. Treatment of HW cells with OGSP significantly attenuated 
      the dysregulated production of adipokines. Moreover, OGSP significantly 
      suppressed coculture-induced production of reactive oxygen species (ROS). 
      Although enhanced release of free fatty acids (FFAs) by coculture was not altered 
      by OGSP, FFA-induced ROS production in HW cells was significantly attenuated by 
      OGSP. Furthermore, OGSP significantly reduced increases in the transcriptional 
      activity of nuclear factor-kappaB and activation of extracellular 
      signal-regulated kinase by coculture. Thus, these results suggest that the 
      antioxidative properties of OGSP attenuate inflammatory changes induced by the 
      coculture of adipocytes and macrophages.
FAU - Sakurai, Takuya
AU  - Sakurai T
AD  - Department of Molecular Predictive Medicine and Sport Science, Kyorin University, 
      School of Medicine, Tokyo 181-8611, Japan.
FAU - Kitadate, Kentaro
AU  - Kitadate K
FAU - Nishioka, Hiroshi
AU  - Nishioka H
FAU - Fujii, Hajime
AU  - Fujii H
FAU - Kizaki, Takako
AU  - Kizaki T
FAU - Kondoh, Yasumasa
AU  - Kondoh Y
FAU - Izawa, Tetsuya
AU  - Izawa T
FAU - Ishida, Hitoshi
AU  - Ishida H
FAU - Radak, Zsolt
AU  - Radak Z
FAU - Ohno, Hideki
AU  - Ohno H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090120
PL  - United States
TA  - J Nutr Biochem
JT  - The Journal of nutritional biochemistry
JID - 9010081
RN  - 0 (Antioxidants)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Flavonoids)
RN  - 0 (Phenols)
RN  - 0 (Plasminogen Activator Inhibitor 1)
RN  - 0 (Polyphenols)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adipocytes/cytology/*physiology
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Cell Death/drug effects
MH  - Cell Line
MH  - Chemokine CCL2/biosynthesis
MH  - Coculture Techniques
MH  - Flavonoids/*pharmacology/therapeutic use
MH  - Inflammation/*drug therapy/pathology
MH  - Macrophages/cytology/*physiology
MH  - Mice
MH  - Oxidative Stress/drug effects
MH  - Phenols/*pharmacology/therapeutic use
MH  - Plasminogen Activator Inhibitor 1/biosynthesis
MH  - Polyphenols
MH  - Seeds/chemistry
MH  - Tumor Necrosis Factor-alpha/biosynthesis
MH  - Vitis/*chemistry
EDAT- 2009/01/23 09:00
MHDA- 2010/02/02 06:00
CRDT- 2009/01/23 09:00
PHST- 2008/05/17 00:00 [received]
PHST- 2008/09/29 00:00 [revised]
PHST- 2008/10/02 00:00 [accepted]
PHST- 2009/01/23 09:00 [entrez]
PHST- 2009/01/23 09:00 [pubmed]
PHST- 2010/02/02 06:00 [medline]
AID - S0955-2863(08)00222-2 [pii]
AID - 10.1016/j.jnutbio.2008.10.003 [doi]
PST - ppublish
SO  - J Nutr Biochem. 2010 Jan;21(1):47-54. doi: 10.1016/j.jnutbio.2008.10.003. Epub 
      2009 Jan 20.