PMID- 19159346
OWN - NLM
STAT- MEDLINE
DCOM- 20090224
LR  - 20160512
IS  - 1447-6959 (Print)
IS  - 1447-073X (Linking)
VI  - 83
IP  - 4
DP  - 2008 Dec
TI  - Immunoelectron microscopic analysis of the distribution of tyrosine kinase 
      receptor B in olfactory axons.
PG  - 186-94
LID - 10.1111/j.1447-073X.2007.00208.x [doi]
AB  - To determine the morphological basis for the neurotrophic effects of 
      brain-derived neurotrophic factor (BDNF) in the primary olfactory pathway (POP), 
      tyrosine kinase receptor B (TrkB), a membrane-bound receptor for BDNF, was 
      identified and localized in axons of olfactory receptor cells (ORC) of neonatal 
      rat olfactory mucosa using immuno-histochemical and -cytochemical techniques. 
      Initially, the immunospecificity of an anti-TrkB antibody that had been used as a 
      specific antibody for full-length TrkB was confirmed in the olfactory mucosa. 
      Then, a combination of a reduced osmium-LR-White and post-embedding immunogold 
      technique was applied to ORC axons in the lamina propria just beneath the 
      olfactory epithelium. Immunogold particles, which indicate TrkB immunoreactivity, 
      were noted either in close association with the plasma membranes of ORC axons, 
      and designated plasma-lemmal (PL), or within their cytoplasm, and designated 
      cytoplasmic (CP). Most PL particles were seen in the CP portion of the axonal 
      plasma membranes, suggesting that the anti-TrkB antibody binds to the 
      membrane-inserted TrkB that acts as a functional receptor. Some CP particles were 
      on vesicular structures. Quantitative analysis demonstrated that the ratio of CP 
      to PL particles was 7:3, and this ratio was constant between animals examined (n 
      = 5). Because membrane proteins are wrapped in vesicles and transported within 
      the axonal cytoplasm and inserted into the plasma membrane to function there, the 
      present study suggests that TrkB is transported within the cytoplasm of ORC axons 
      and is positioned as a functional receptor for BDNF in their membranes.
FAU - Hasegawa, Rumi
AU  - Hasegawa R
AD  - Laboratory of Anatomy, Faculty and Graduate School of Health Sciences, Kyorin 
      University, Tokyo, Japan.
FAU - Takami, Shigeru
AU  - Takami S
FAU - Nishiyama, Fumiaki
AU  - Nishiyama F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Anat Sci Int
JT  - Anatomical science international
JID - 101154140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Antibody Specificity
MH  - Axons/*metabolism/*ultrastructure
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cytoplasm/metabolism/ultrastructure
MH  - Female
MH  - Male
MH  - Microscopy, Immunoelectron/methods
MH  - Olfactory Mucosa/metabolism/ultrastructure
MH  - Olfactory Nerve/*metabolism/*ultrastructure
MH  - Olfactory Receptor Neurons/metabolism/ultrastructure
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/*metabolism
EDAT- 2009/01/23 09:00
MHDA- 2009/02/25 09:00
CRDT- 2009/01/23 09:00
PHST- 2009/01/23 09:00 [entrez]
PHST- 2009/01/23 09:00 [pubmed]
PHST- 2009/02/25 09:00 [medline]
AID - ASI208 [pii]
AID - 10.1111/j.1447-073X.2007.00208.x [doi]
PST - ppublish
SO  - Anat Sci Int. 2008 Dec;83(4):186-94. doi: 10.1111/j.1447-073X.2007.00208.x.