PMID- 19159567
OWN - NLM
STAT- MEDLINE
DCOM- 20090611
LR  - 20161124
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 88
IP  - 48
DP  - 2008 Dec 30
TI  - [Immunoglobulin domain and mucin domain-1 in helper T lymphocytes in allergic 
      rhinitis].
PG  - 3392-6
AB  - OBJECTIVE: To investigate the significance of T cell immunoglobulin domain and 
      mucin domain-1 (TIM-1) expression in helper T lymphocyte in allergic rhinitis 
      (AR) patients. METHODS: Peripheral blood samples were collected from 20 AR 
      patients, 9 males and 11 females, aged 31.4 (20-54), and 20 healthy persons, 12 
      males and 8 females, aged 38, 6 (18-60). Peripheral blood mononuclear cells 
      (PBMNs) were isolated and magnetic cell sorting technique was used to purify the 
      CD4+ T cells and CD4 + CD45RA + T cells. Immunofluorescence staining and flow 
      cytometry were used to detect the percentage of CD4 + CD45RA + T cells positive 
      in TIM-1, a helper T lymphocyte marker. Phytohemagglutinin (PHA) was added into 
      the culture media to stimulate the TIM-1 positive helper T lymphocytes. Dendritic 
      cells (DCs) thus induced and differentiated were conditioned by standardized 
      allergen from dust mite (Dermatophagoides pteronyssinus) or lipopolysaccharide 
      (LPS), and then co-cultured with CD4 T cells. The percentage of TIM-1 positive 
      CD4 + CD45RA + T cells was counted. ELISA was used to detect the IL-4 and 
      interferon (IFN)-gamma levels in the supernatants of the culture media of the 
      dust mite and LPS groups. RESULTS: The percentage of TIM-1 positive CD4+ T cells 
      of the AR patients was (9.48 +/- 1.51)%, significantly higher than that of the 
      healthy controls [(3.12 +/- 0.32)%, P < 0.05], and the percentage of TIM-1 
      positive CD4 + T cells after PHA stimulation of the AR patients was (47.05 +/- 
      4.35)%, significantly higher than that of the healthy controls [(29.92 +/- 
      3.36)%, P < 0.05]. The percentage of TIM-1 positive CD4 + CD45RA + T cells of the 
      AR patients was (26.34 +/- 4.0)%, significantly higher than that of the healthy 
      controls [(18.4 +/- 8.1)%, P < 0.05], and the percentage of TIM-1 positive CD4 + 
      CD45RA + T cells after PHA stimulation of the AR patients was (65.3 +/- 7.4)%, 
      significantly higher than that of the healthy controls [(36.3 +/- 6.8)%, P < 
      0.05]. The percentage of TIM-1 positive CD4 + CD45RA + T cells co-cultured with 
      the DCs conditioned by dust mite of the AR patients was (10.81 +/- 1.48)%, 
      significantly higher than that of the LPS group [(6.08 +/- 1.45)%] and the blank 
      control group [(1.56 +/- 2.30)%, both P < 0.05]. The IL-4 level of the DCs 
      conditioned by dust mite allergen was (74.61 +/- 13.82) pg/ml, significantly 
      higher than those of the LPS group and blank control group [(28.57 +/- 3.36) and 
      (1.40 +/- 0.99) ng/ml respectively, both P < 0.05]. The IFN-gamma level of the 
      DCs conditioned by dust mite allergen was (25.31 +/- 7.23) pg/ml, significantly 
      lower than that of the LPS group [(163.41 +/- 82.37) pg/ml, P < 0.05], however, 
      significantly higher than that of the blank control group [(1.58 +/- 0.53) pg/ml, 
      P < 0.05]. CONCLUSION: The percentage of TIM-1 positive helper T lymphocytes of 
      the AR patients are abnormally high. After stimulation of allergen The DCs 
      increase the number of TIM-1 expressing naive Th cells and promote them to 
      differentiate into Th2 cells. Promoting naive Th cells to differentiate into Th2 
      cells, TIM-1 expression induces Th2 cytokine-based immune responses.
FAU - Wu, Wen-kui
AU  - Wu WK
AD  - Department of Otolaryngology, Second Hospital of Shanxi Medical University, 
      Taiyuan 030001, China.
FAU - An, Yun-fang
AU  - An YF
FAU - Zhao, Chang-qing
AU  - Zhao CQ
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (HAVCR1 protein, human)
RN  - 0 (Hepatitis A Virus Cellular Receptor 1)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Virus)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Cell Differentiation
MH  - Dendritic Cells/immunology
MH  - Female
MH  - Flow Cytometry
MH  - Hepatitis A Virus Cellular Receptor 1
MH  - Humans
MH  - Male
MH  - Membrane Glycoproteins/*metabolism
MH  - Middle Aged
MH  - Receptors, Virus/*metabolism
MH  - Rhinitis, Allergic, Perennial/*immunology/metabolism
MH  - T-Lymphocyte Subsets/immunology/*metabolism
MH  - Th2 Cells/immunology/*metabolism
EDAT- 2009/01/23 09:00
MHDA- 2009/06/12 09:00
CRDT- 2009/01/23 09:00
PHST- 2009/01/23 09:00 [entrez]
PHST- 2009/01/23 09:00 [pubmed]
PHST- 2009/06/12 09:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2008 Dec 30;88(48):3392-6.