PMID- 19159786
OWN - NLM
STAT- MEDLINE
DCOM- 20090227
LR  - 20131121
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 77
IP  - 5
DP  - 2009 Mar 15
TI  - Rapid determination of Delta9-Tetrahydrocannabinol in saliva by polymer monolith 
      microextraction combined with gas chromatography-mass spectrometry.
PG  - 1701-6
LID - 10.1016/j.talanta.2008.10.007 [doi]
AB  - A method was developed for the determination of Delta(9)-Tetrahydrocannabinol 
      (THC) in saliva by polymer monolith microextraction (PMME) combined with gas 
      chromatography-mass spectrometry. The poly(methacrylic acid-co-ethylene glycol 
      dimethacrylate) (p(MAA-co-EGDMA)) monolithic capillary column was selected as the 
      extraction medium of PMME, which showed high extraction capacity towards THC in 
      saliva. To reach optimum PMME extraction performance, several PMME parameters 
      were investigated, including matrix pH, flow rate for extraction, sampling volume 
      and elution solvent. Under the optimal conditions, good extraction efficiency was 
      obtained with no matrix interference in the process of extraction and the 
      subsequent GC-MS analysis. In the selected-ion monitoring (SIM) mode, the limit 
      of detection (LOD) for THC was 0.68 ng/mL. The linearity range of the method was 
      3-300 ng/mL. Excellent reproducibility of the method was exhibited by intra- and 
      inter-day precisions, yielding the relative standard deviations (R.S.D.s) less 
      than 12%; recoveries higher than 89%. The proposed method was proved to be rapid, 
      sensitive, and competently applied to the determination of THC in saliva samples.
FAU - Luo, Dan
AU  - Luo D
AD  - Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Chen, Fei
AU  - Chen F
FAU - Xiao, Kuang
AU  - Xiao K
FAU - Feng, Yu-Qi
AU  - Feng YQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081017
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Psychotropic Drugs)
RN  - 7J8897W37S (Dronabinol)
SB  - IM
MH  - Dronabinol/*analysis
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Humans
MH  - Methods
MH  - Psychotropic Drugs/analysis
MH  - *Saliva
EDAT- 2009/01/23 09:00
MHDA- 2009/02/28 09:00
CRDT- 2009/01/23 09:00
PHST- 2008/06/28 00:00 [received]
PHST- 2008/10/01 00:00 [revised]
PHST- 2008/10/02 00:00 [accepted]
PHST- 2009/01/23 09:00 [entrez]
PHST- 2009/01/23 09:00 [pubmed]
PHST- 2009/02/28 09:00 [medline]
AID - S0039-9140(08)00729-7 [pii]
AID - 10.1016/j.talanta.2008.10.007 [doi]
PST - ppublish
SO  - Talanta. 2009 Mar 15;77(5):1701-6. doi: 10.1016/j.talanta.2008.10.007. Epub 2008 
      Oct 17.