PMID- 19160024
OWN - NLM
STAT- MEDLINE
DCOM- 20090727
LR  - 20211020
IS  - 1573-4838 (Electronic)
IS  - 0957-4530 (Linking)
VI  - 20
IP  - 6
DP  - 2009 Jun
TI  - Stability of prostaglandin E(2) (PGE (2)) embedded in poly-D,L: 
      -lactide-co-glycolide microspheres: a pre-conditioning approach for tissue 
      engineering applications.
PG  - 1357-65
LID - 10.1007/s10856-008-3678-9 [doi]
AB  - Prostaglandin E(2) (PGE(2)) is involved in angiogenesis, bone repair and 
      cartilage metabolism. Thus, PGE(2) might represent a suitable signaling molecule 
      in different tissue engineering applications. PGE(2) also has a short half-life 
      time. Its incorporation into poly-D: ,L: -lactide-co-glycolide (PLGA) 
      microspheres was demonstrated in a previous study. However, the stability of 
      bioactive PGE(2) in these microspheres is unknown. With an adjusted mass 
      spectrometry assay we investigated the amount of incorporated PGE(2) and the 
      stability of PGE(2) in conventional cell culture medium and in PLGA microspheres. 
      The stability of PGE(2) was closely pH dependent. Strong acidic or basic 
      environments reduced the half-life from 300 h (pH 2.6-4.0) to below 50 h at pH 
      2.0 or pH 8.8. The half-life of PGE(2) incorporated into poly-D: ,L: 
      -lactide-co-glycolide increased drastically to 70 days at 37 degrees C and to 300 
      days at 8 degrees C. Analysis with scanning electron microscopy (SEM) and atomic 
      force microscopy (AFM) demonstrated a distinct nanostructure of the polymeric 
      phase and both nano- and microporosity.
FAU - Watzer, Bernhard
AU  - Watzer B
AD  - Mother-Child Medical Center, Department of Pediatric Science, 
      Philipps-University, Marburg, Germany.
FAU - Zehbe, Rolf
AU  - Zehbe R
FAU - Halstenberg, Sven
AU  - Halstenberg S
FAU - James Kirkpatrick, C
AU  - James Kirkpatrick C
FAU - Brochhausen, Christoph
AU  - Brochhausen C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090122
PL  - United States
TA  - J Mater Sci Mater Med
JT  - Journal of materials science. Materials in medicine
JID - 9013087
RN  - 0 (Drug Carriers)
RN  - 0 (Polymers)
RN  - 059QF0KO0R (Water)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Chromatography, Liquid
MH  - Dinoprostone/*chemistry
MH  - Drug Carriers/chemistry
MH  - Drug Stability
MH  - Gas Chromatography-Mass Spectrometry
MH  - Half-Life
MH  - Lactic Acid/*chemistry
MH  - Mass Spectrometry
MH  - Microscopy, Atomic Force
MH  - Microscopy, Electron, Scanning
MH  - *Microspheres
MH  - Nanoparticles/chemistry/ultrastructure
MH  - Particle Size
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*chemistry
MH  - Porosity
MH  - Temperature
MH  - Tissue Engineering/*methods
MH  - Water/chemistry
EDAT- 2009/01/23 09:00
MHDA- 2009/07/28 09:00
CRDT- 2009/01/23 09:00
PHST- 2008/11/25 00:00 [received]
PHST- 2008/12/16 00:00 [accepted]
PHST- 2009/01/23 09:00 [entrez]
PHST- 2009/01/23 09:00 [pubmed]
PHST- 2009/07/28 09:00 [medline]
AID - 10.1007/s10856-008-3678-9 [doi]
PST - ppublish
SO  - J Mater Sci Mater Med. 2009 Jun;20(6):1357-65. doi: 10.1007/s10856-008-3678-9. 
      Epub 2009 Jan 22.