PMID- 19160748
OWN - NLM
STAT- MEDLINE
DCOM- 20100624
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 26
IP  - 5
DP  - 2008 Sep
TI  - [Identification of Mycobacterium species using reversed-phase high performance 
      liquid chromatographic analysis of mycolic acid].
PG  - 534-9
AB  - A method for the identification of Mycobacterium species using reversed-phase 
      high performance liquid chromatography (RP-HPLC) was developed. The fingerprints 
      library was constructed on the basis of RP-HPLC chromatograms of the mycolic 
      acids derivatives from 49 Mycobacterium species cultures. Two inoculation loops 
      of Mycobacterium, for fast growth with one week incubation or for slow growth 
      with three weeks incubation, were saponified for 1 h and stocked at 4 degrees C. 
      The mycolic acids from each culture of Mycobacterium species were acidified, 
      extracted, derivatized, and analyzed by the RP-HPLC method. On the basis of the 
      HPLC patterns of relative retention time and relative peak height, the 
      identifications of Mycobacterium species were performed. This established method 
      has a good precision of retention times with the relative standard deviations 
      (RSDs) ranging from 0.13% to 1.07%. The mycolic acids fingerprints library of 
      HPLC patterns was set up, including 49 species that were recorded in "Bergey's 
      Manual of Systematic Bacteriology". Three patterns were observed from the 
      chromatographic behaviors of mycolic acids derivatives, including single 
      peak-cluster, double peak-clusters, triple and multiple peak-clusters. Forty-one 
      species were successfully identified according to the different relative 
      retention times of the peaks and the relative peak heights. The established 
      method can identify Mycobacterium species with rapidity and high reliability.
FAU - Du, Rong
AU  - Du R
AD  - Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
FAU - Chen, Baowen
AU  - Chen B
FAU - Guo, Lei
AU  - Guo L
FAU - Li, Yang
AU  - Li Y
FAU - Xie, Jianwei
AU  - Xie J
FAU - Wang, Guozhi
AU  - Wang G
FAU - Zhou, Hongbing
AU  - Zhou H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Mycolic Acids)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Chromatography, Reverse-Phase/*methods
MH  - Mycobacterium/*chemistry/*classification
MH  - Mycolic Acids/*analysis/chemistry
MH  - Reproducibility of Results
EDAT- 2009/01/24 09:00
MHDA- 2010/06/25 06:00
CRDT- 2009/01/24 09:00
PHST- 2009/01/24 09:00 [entrez]
PHST- 2009/01/24 09:00 [pubmed]
PHST- 2010/06/25 06:00 [medline]
AID - 10.1016/s1872-2059(08)60027-9 [doi]
PST - ppublish
SO  - Se Pu. 2008 Sep;26(5):534-9. doi: 10.1016/s1872-2059(08)60027-9.