PMID- 19160763
OWN - NLM
STAT- MEDLINE
DCOM- 20100624
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 26
IP  - 5
DP  - 2008 Sep
TI  - [Pyrolysis-gas chromatographic fingerprints with hierarchical cluster analysis 
      for Dendrobium candidum Wall. ex Lindl].
PG  - 613-7
AB  - The pyrogram fingerprints of Dendrobium candidum Wall. ex Lindl. from different 
      regions were studied by pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) 
      and compared with hierarchical cluster analysis. The effect of pyrolysis 
      temperature on the fingerprint was examined by evolved gas analysis, and then 450 
      degrees C was selected as the optimized pyrolysis temperature. An amount of 0.4 
      mg of raw drug powder was pyrolysed in a vertical microfurnace pyrolyzer, and the 
      products were directly introduced into a gas chromatograph equipped with a flame 
      ionization detector (FID) and a fused-silica capillary column (30 m x 0.25 mm x 
      0.25 microm). The pyrogram fingerprints of 10 samples from different regions 
      showed a high similarity and a good reproducibility with the relative standard 
      deviations (RSDs) of the retention times less than 0.33% and the RSDs of the 
      relative peak areas less than 4.8%. Therefore, each sample was characterized by 
      the peak area of 31 peaks in each pyrogram and these peaks were employed for 
      hierarchical cluster analysis. Furthermore, the discrimination of the sample from 
      different regions was achieved by hierarchical cluster analysis via recognizing 
      the 10 x 31 data matrix. Thus, the results proved it is a simple, rapid and 
      accurate method suitable for the quality control of the traditional Chinese 
      medicines.
FAU - Wang, Lili
AU  - Wang L
AD  - College of Chemical Engineering and Materials Science, Zhejiang University of 
      Technology, Hangzhou 310014, China. lili_wang@zjut.edu.cn
FAU - Wang, Cong
AU  - Wang C
FAU - Pan, Zaifa
AU  - Pan Z
FAU - Sun, Fa
AU  - Sun F
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
SB  - IM
MH  - Cluster Analysis
MH  - Dendrobium/*chemistry/classification
MH  - Gas Chromatography-Mass Spectrometry/*methods/statistics & numerical data
MH  - Quality Control
MH  - Reproducibility of Results
MH  - Temperature
MH  - Time Factors
EDAT- 2009/01/24 09:00
MHDA- 2010/06/25 06:00
CRDT- 2009/01/24 09:00
PHST- 2009/01/24 09:00 [entrez]
PHST- 2009/01/24 09:00 [pubmed]
PHST- 2010/06/25 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2008 Sep;26(5):613-7.