PMID- 19160840
OWN - NLM
STAT- MEDLINE
DCOM- 20100129
LR  - 20131121
IS  - 1000-3061 (Print)
IS  - 1000-3061 (Linking)
VI  - 24
IP  - 9
DP  - 2008 Sep
TI  - [Construction and application of chimeric infectious clones of porcine 
      reproductive and respiratory syndrome virus].
PG  - 1573-81
AB  - In recent years, mass outbreaks of highly pathogenic (HP) porcine reproductive 
      and respiratory syndrome virus (PRRSV) have spread all over the Chinese swine 
      industry. Based on the first infectious cDNA clone of HP PRRSV strain pJX143 and 
      that of an attenuated PRRSV, pAPRRS, constructed in our group, we constructed 
      several chimeric clones with various substitutions of structural protein genes 
      (ORF4-7) and 3' UTR between attenuated pAPRRS and virulent pJX143.Upon 
      transfection of MA-104 cultured cells, all chimeric constructs pSX12, p5NX12, and 
      p56N12 were rescued. The rescued viruses maintained the similar virological 
      properties, based on the results of the growth curve of the rescued viruses. To 
      test if the chimeric viruses can be used as a vaccine candidate, vSX12 and v56N12 
      vaccinated pigs were challenged with the HP PRRSV JX143 strain. As a result, the 
      vSX12 vaccinated pigs were all seroconverted by 14-day-post vaccination, while 
      v56N12 vaccinated pigs showed poor antibody response. Upon challenge, the 
      vSX12-vaccinated group showed no signs of clinical PRRS syndrome, and virema 
      period was shorten to 6 days post-challenge. Our results demonstrated that 1) 
      vSX12 chimeric virus is a good vaccine candidate; 2) the virulence determinants 
      of HP PRRSV probably located in coding regions other than ORF3-7 and 3' UTR, as 
      our chimeric viruses were proved to be attenuated.
FAU - Li, Xiangjian
AU  - Li X
AD  - Department of Animal Infectious Diseases, Shanghai Veterinary Research Institute, 
      China Academy of Agricultural Sciences, Shanghai 200241, China.
FAU - Zhang, Jianwu
AU  - Zhang J
FAU - Lu, Jian
AU  - Lu J
FAU - Yu, Dandan
AU  - Yu D
FAU - Yao, Huochun
AU  - Yao H
FAU - Yuan, Shishan
AU  - Yuan S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Cloning, Molecular
MH  - *Open Reading Frames
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/immunology
MH  - Recombinant Fusion Proteins/genetics/immunology
MH  - Recombination, Genetic/genetics
MH  - Swine
MH  - Vaccines, Attenuated/immunology
MH  - Viral Envelope Proteins
MH  - Viral Proteins/biosynthesis/*genetics
MH  - Viral Vaccines/immunology
EDAT- 2009/01/24 09:00
MHDA- 2010/01/30 06:00
CRDT- 2009/01/24 09:00
PHST- 2009/01/24 09:00 [entrez]
PHST- 2009/01/24 09:00 [pubmed]
PHST- 2010/01/30 06:00 [medline]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2008 Sep;24(9):1573-81.