PMID- 19162463
OWN - NLM
STAT- MEDLINE
DCOM- 20090526
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 24
IP  - 8
DP  - 2009 Apr 15
TI  - Passaged neural stem cell-derived neuronal networks for a portable biosensor.
PG  - 2365-70
LID - 10.1016/j.bios.2008.12.007 [doi]
AB  - We have previously demonstrated a portable biosensor that utilizes networks of 
      mammalian neurons on microelectrode arrays (MEAs) as the sensing element. These 
      neuronal cultures on MEAs are derived from primary neuronal tissues and are 
      short-lived. In order to extend the shelf life of neuronal networks for use in a 
      fieldable sensor technology, a renewable source of networks is needed. Neural 
      stem and progenitor cells are capable of self-renewal and differentiation into 
      functional neuronal networks. The purpose of this study was to develop a strategy 
      for growing passaged neural stem and progenitor cells on MEAs under controlled 
      conditions to produce differentiated neurons and glia comprising functional 
      neuronal networks. Primary and passaged neuroepithelial stem and progenitor cells 
      dissociated from embryonic day 13 rat cortex were seeded on MEAs and maintained 
      with serum-free medium containing basic fibroblast growth factor (bFGF) combined 
      with brain-derived neurotrophic factor (BDNF). These culture conditions lead to 
      abundant neurons, with astrocytes as supportive cells, forming synaptically 
      linked networks of neurons. Spontaneous action potentials were best recorded from 
      networks derived from primary or passaged progenitor cells 4-5 weeks after 
      initial culture. The passaged progenitor cell-derived networks on MEAs responded 
      to the GABA(A) antagonist bicuculline, the NMDA glutamate inhibitor APV, and the 
      non-NMDA glutamate antagonist CNQX indicating active synapses were present. 
      Passaged neural stem and progenitor cell-derived networks on MEAs have properties 
      similar to networks derived from primary neuronal cultures and can serve as a 
      renewable supply of sensor elements for detection of environmental threats.
FAU - O'Shaughnessy, Thomas J
AU  - O'Shaughnessy TJ
AD  - Center for Bio/Molecular Science and Engineering, Code 6900, Naval Research 
      Laboratory, Washington, DC 20375, USA. thomas.oshaughnessy@nrl.navy.mil
FAU - Liu, Jinny L
AU  - Liu JL
FAU - Ma, Wu
AU  - Ma W
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20081209
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
SB  - IM
MH  - Action Potentials/physiology
MH  - Animals
MH  - Biological Assay/*instrumentation/methods
MH  - Biosensing Techniques/*instrumentation
MH  - Cell Culture Techniques/instrumentation/methods
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Nerve Net/anatomy & histology/*physiology
MH  - Neurons/*cytology/*physiology
MH  - Rats
MH  - Stem Cells/*cytology/*physiology
EDAT- 2009/01/24 09:00
MHDA- 2009/05/27 09:00
CRDT- 2009/01/24 09:00
PHST- 2008/09/11 00:00 [received]
PHST- 2008/11/14 00:00 [revised]
PHST- 2008/12/03 00:00 [accepted]
PHST- 2009/01/24 09:00 [entrez]
PHST- 2009/01/24 09:00 [pubmed]
PHST- 2009/05/27 09:00 [medline]
AID - S0956-5663(08)00646-5 [pii]
AID - 10.1016/j.bios.2008.12.007 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2009 Apr 15;24(8):2365-70. doi: 10.1016/j.bios.2008.12.007. 
      Epub 2008 Dec 9.