PMID- 19164786
OWN - NLM
STAT- MEDLINE
DCOM- 20090424
LR  - 20210105
IS  - 1524-4628 (Electronic)
IS  - 0039-2499 (Linking)
VI  - 40
IP  - 4
DP  - 2009 Apr
TI  - Brain-derived neurotrophic factor contributes to recovery of skilled reaching 
      after focal ischemia in rats.
PG  - 1490-5
LID - 10.1161/STROKEAHA.108.531806 [doi]
AB  - BACKGROUND AND PURPOSE: Brain-derived neurotrophic factor (BDNF) is involved in 
      neuronal survival, synaptic plasticity, learning and memory, and neuroplasticity. 
      Further, exogenous treatment with BDNF or exposing animals to enrichment and 
      exercise regimens, which also increase BDNF, enhances behavioral recovery after 
      brain injury. Thus, the beneficial effects of rehabilitation in promoting 
      recovery after stroke may also depend on BDNF. We tested this hypothesis by 
      evaluating the contribution of BDNF to motor skill relearning after 
      endothelin-1-induced middle cerebral artery occlusion in rats. METHODS: Antisense 
      BDNF oligonucleotide, which blocks the expression of BDNF (or saline vehicle) was 
      infused into the contralateral lateral ventricle for 28 days after ischemia. 
      Animals received either a graduated rehabilitation program, including running 
      exercise and skilled reaching training, which simulates clinical practice, or no 
      rehabilitation. Functional recovery was assessed with a battery of tests that 
      measured skilled reaching, forelimb use asymmetry, and foraging ability. RESULTS: 
      Rehabilitation significantly improved skilled reaching ability in the staircase 
      task. Antisense BDNF oligonucleotide effectively blocked BDNF mRNA, and negated 
      the beneficial effects of rehabilitation on recovery of skilled reaching. 
      Importantly, antisense BDNF oligonucleotide did not affect reaching with the 
      unaffected limb, body weight, infarct size, or foraging ability, indicating the 
      treatment was specific to relearning of motor skill after ischemia. CONCLUSIONS: 
      This study is the first to identify a critical role for BDNF in 
      rehabilitation-induced recovery after stroke, and our results suggest that new 
      treatments to enhance BDNF would constitute a promising therapy for promoting 
      recovery of function after stroke.
FAU - Ploughman, Michelle
AU  - Ploughman M
AD  - Division of BioMedical Sciences, Faculty of Medicine, Memorial University of 
      Newfoundland, St John's, NL, Canada.
FAU - Windle, Victoria
AU  - Windle V
FAU - MacLellan, Crystal L
AU  - MacLellan CL
FAU - White, Nicole
AU  - White N
FAU - Dore, Jules J
AU  - Dore JJ
FAU - Corbett, Dale
AU  - Corbett D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090122
PL  - United States
TA  - Stroke
JT  - Stroke
JID - 0235266
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Oligoribonucleotides, Antisense)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Brain Ischemia/pathology/*physiopathology/rehabilitation
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Exercise Therapy
MH  - Forelimb/physiology
MH  - Infarction, Middle Cerebral Artery/pathology/physiopathology/rehabilitation
MH  - Male
MH  - Motor Skills/*physiology
MH  - Oligoribonucleotides, Antisense/pharmacology
MH  - Posture
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function/*physiology
MH  - Running
EDAT- 2009/01/24 09:00
MHDA- 2009/04/25 09:00
CRDT- 2009/01/24 09:00
PHST- 2009/01/24 09:00 [entrez]
PHST- 2009/01/24 09:00 [pubmed]
PHST- 2009/04/25 09:00 [medline]
AID - STROKEAHA.108.531806 [pii]
AID - 10.1161/STROKEAHA.108.531806 [doi]
PST - ppublish
SO  - Stroke. 2009 Apr;40(4):1490-5. doi: 10.1161/STROKEAHA.108.531806. Epub 2009 Jan 
      22.