PMID- 19166213
OWN - NLM
STAT- MEDLINE
DCOM- 20100128
LR  - 20090126
IS  - 1001-5515 (Print)
IS  - 1001-5515 (Linking)
VI  - 25
IP  - 6
DP  - 2008 Dec
TI  - [The neuroprotective role of brain-derived neurotrophic factor for embryonic rat 
      cortical neurons against hypoxia via CREB phosphorylation].
PG  - 1377-80
AB  - Transcription factor cyclic AMP response element-binding protein (CREB) in 
      embryonic cortical neurons is an important modulator of Brain-derived 
      neurotrophic factor (BDNF) induced gene expression. Meanwhile, our early 
      researches have indicated that BDNF possesses neuroprotective role for hypoxic 
      neurons against hypoxia. In order todisclose whether the neuroprotective role of 
      BDNF for embryonic rat cortical neurons against hypoxia is fulfilled via 
      nucleoprotein CREB phosphorylation, we used western blotting method to detect the 
      expression of CREB and phosphorylated CREB in experimental groups (with BDNF) and 
      hypoxic control group (without BDNF) with the time changes of exposure to 
      hypoxia. Results indicated that hypoxia and BDNF both could induce 
      phosphorylation of CREB in embryonic cortical neurons. Phosphorylation of CREB in 
      experimental group (with BDNF) was much higher than that in hypoxic control group 
      at the same time points (P<0.01). The expression of phosphorylated CREB reached 
      the highest level at the first hour after being exposed to hypoxia in 
      experimental groups, then phosphorylated CREB decreased slowly and remained at 
      the level for much longer time in experimental groups than in control group. The 
      total amount of CREB in embryonic cortical neurons at the first 0-3 hours after 
      being exposed to hypoxia in experimental groups were the same as that in hypoxic 
      control group. CREB decreased more quickly in hypoxic control group at 5-6 hours 
      after hypoxia. This in vitro research demonstrates that BDNF plays its 
      neuroprotective role for embryonic rat cortical neurons against hypoxia via CREB 
      phosphorylation.
FAU - Luo, Xiaoli
AU  - Luo X
AD  - Department of Pediatrics, West China Second University Hospital, Sichuan 
      University, Chengdu 610041, China.
FAU - Zhou, Hui
AU  - Zhou H
FAU - Sun, Xiaomei
AU  - Sun X
FAU - Li, Shengfu
AU  - Li S
FAU - Mu, Dezhi
AU  - Mu D
FAU - Mao, Meng
AU  - Mao M
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
JT  - Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = 
      Shengwu yixue gongchengxue zazhi
JID - 9426398
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Neuroprotective Agents)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/chemistry/*pharmacology
MH  - Cell Hypoxia
MH  - Cells, Cultured
MH  - Cerebral Cortex/*cytology
MH  - Cyclic AMP Response Element-Binding Protein/*chemistry
MH  - Embryo, Mammalian
MH  - Neurons/*cytology
MH  - Neuroprotective Agents/chemistry/*pharmacology
MH  - Phosphorylation
MH  - Rats
EDAT- 2009/01/27 09:00
MHDA- 2010/01/29 06:00
CRDT- 2009/01/27 09:00
PHST- 2009/01/27 09:00 [entrez]
PHST- 2009/01/27 09:00 [pubmed]
PHST- 2010/01/29 06:00 [medline]
PST - ppublish
SO  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2008 Dec;25(6):1377-80.