PMID- 19168037
OWN - NLM
STAT- MEDLINE
DCOM- 20090421
LR  - 20240319
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1259
DP  - 2009 Mar 9
TI  - Estradiol increases Pet-1 and serotonin transporter mRNA in the midbrain raphe 
      nuclei of ovariectomized rats.
PG  - 51-8
LID - 10.1016/j.brainres.2008.12.067 [doi]
AB  - Previous research has shown that estradiol increases the anorexia associated with 
      serotonin (5-HT) neurotransmission. To examine further the putative relationship 
      between estradiol and 5-HT, we investigated whether estradiol increases the 
      expression of Pet-1 and the 5-HT transporter (5-HTT), two genes implicated in the 
      development and regulation of the 5-HT system. Ovariectomized (OVX) rats 
      (n=5-6/group) were treated with 0, 2, or 10 microg estradiol benzoate (EB) in 
      sesame oil on 2 consecutive days. Food intake and body weight were recorded 2 
      days later when EB-treated rats typically display signs of behavioral estrus 
      (e.g., reduced feeding). Following the collection of behavioral data, rats were 
      perfused, brains were removed, and coronal sections were cut through the midbrain 
      raphe nuclei. Pet-1 and 5-HTT mRNA levels were quantified throughout the dorsal 
      and median raphe nuclei (DRN and MRN) by conducting in situ hybridization on 
      free-floating tissue sections using (35)S-labeled cDNA probes. As expected, EB 
      treatment decreased food intake and body weight on the day that modeled estrus. 
      At this same time, EB treatment increased Pet-1 and 5-HTT mRNA levels within the 
      DRN and MRN. We conclude that a physiologically relevant regimen of estradiol 
      treatment in OVX rats increases Pet-1 and 5-HTT mRNA levels in the midbrain raphe 
      nuclei at a time when the anorexigenic effect of estradiol is apparent. Further 
      studies are required to determine whether the increased expression of Pet-1 and 
      5-HTT mRNA plays a causal role in the anorexigenic effect of estradiol.
FAU - Rivera, Heidi M
AU  - Rivera HM
AD  - Department of Psychology and Program in Neuroscience, Florida State University, 
      Tallahassee, FL 32306-4301, USA.
FAU - Oberbeck, Denesa R
AU  - Oberbeck DR
FAU - Kwon, Bumsup
AU  - Kwon B
FAU - Houpt, Thomas A
AU  - Houpt TA
FAU - Eckel, Lisa A
AU  - Eckel LA
LA  - eng
GR  - DK-073936/DK/NIDDK NIH HHS/United States
GR  - DC-03198/DC/NIDCD NIH HHS/United States
GR  - R01 DK073936/DK/NIDDK NIH HHS/United States
GR  - R01 DC003198/DC/NIDCD NIH HHS/United States
GR  - T32 DC-00044/DC/NIDCD NIH HHS/United States
GR  - R01 DK073936-03/DK/NIDDK NIH HHS/United States
GR  - T32 DC000044/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090110
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Appetite Depressants)
RN  - 0 (Fev protein, rat)
RN  - 0 (RNA, Messenger)
RN  - 0 (Serotonin Plasma Membrane Transport Proteins)
RN  - 0 (Slc6a4 protein, rat)
RN  - 0 (Transcription Factors)
RN  - 1S4CJB5ZGN (estradiol 3-benzoate)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Appetite Depressants/administration & dosage/pharmacology
MH  - Body Weight
MH  - Eating/drug effects
MH  - Estradiol/administration & dosage/*analogs & derivatives/pharmacology
MH  - Estrus/drug effects
MH  - Female
MH  - Gene Expression/drug effects
MH  - In Situ Hybridization
MH  - *Ovariectomy
MH  - RNA, Messenger/genetics/metabolism
MH  - Raphe Nuclei/drug effects/*metabolism
MH  - Rats
MH  - Rats, Long-Evans
MH  - Serotonin Plasma Membrane Transport Proteins/genetics/*metabolism
MH  - Transcription Factors/genetics/*metabolism
PMC - PMC2957819
MID - NIHMS88685
EDAT- 2009/01/27 09:00
MHDA- 2009/04/22 09:00
PMCR- 2010/10/20
CRDT- 2009/01/27 09:00
PHST- 2008/09/12 00:00 [received]
PHST- 2008/12/29 00:00 [revised]
PHST- 2008/12/31 00:00 [accepted]
PHST- 2009/01/27 09:00 [entrez]
PHST- 2009/01/27 09:00 [pubmed]
PHST- 2009/04/22 09:00 [medline]
PHST- 2010/10/20 00:00 [pmc-release]
AID - S0006-8993(09)00010-9 [pii]
AID - 10.1016/j.brainres.2008.12.067 [doi]
PST - ppublish
SO  - Brain Res. 2009 Mar 9;1259:51-8. doi: 10.1016/j.brainres.2008.12.067. Epub 2009 
      Jan 10.