PMID- 19168069
OWN - NLM
STAT- MEDLINE
DCOM- 20100416
LR  - 20131121
IS  - 1879-0135 (Electronic)
IS  - 0020-7519 (Linking)
VI  - 39
IP  - 7
DP  - 2009 Jun
TI  - Time profiles of the expression of metalloproteinases, tissue inhibitors of 
      metalloproteases, cytokines and collagens in hamsters infected with Opisthorchis 
      viverrini with special reference to peribiliary fibrosis and liver injury.
PG  - 825-35
LID - 10.1016/j.ijpara.2008.12.002 [doi]
AB  - The liver fluke Opisthorchis viverrini is endemic in southeastern Asia, and 
      causes cholangiocarcinoma and liver fibrosis. We investigated the time profile of 
      the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs 
      (TIMPs) in relation to peribiliary fibrosis in O. viverrini-infected hamsters. 
      Hepatic mRNA expression of MMPs, TIMPs, cytokines and collagens I and III was 
      assessed by quantitative reverse transcription-PCR. Zymography and 
      immunohistochemistry were also used to examine MMPs-2 and -9 expression. After 
      infection, an increase of peribiliary fibrosis was time-dependent. Opisthorhis 
      viverrini-induced gene expression in hamster liver, with increased mRNA 
      expression levels of IL-1beta, TNF-alpha, TGF-beta, and collagens I and III, was 
      observed at 21 days p.i. Expression of MMPs-2, -13 and -14 and TIMPs-1 and -3 
      genes, was significantly higher at 1 month, and maximal levels of most MMPs 
      (MMPs-2, -9, -13 and -14) were observed at 2 months p.i. The cytoplasmic levels 
      of MMP-2 and MMP-9 were similar to mRNA expression. Immunohistochemistry revealed 
      that MMP-9 was expressed mainly in the cytoplasm of inflammatory cells at the 
      invasive front of the fibrous area. In contrast, the highest levels of mRNA 
      expression of TIMPs-2 and -3, and TGF-beta were observed 10 months p.i. 
      Concentration of TIMP-2 protein in the plasma correlated with its transcriptional 
      level (r=0.320, P=0.040). Peribiliary fibrosis correlated positively with liver 
      hydroxyproline content (r=0.846, P<0.001), plasma hydroxyproline concentration 
      (r=0.770, P<0.001), plasma TIMP-2 level (r=0.335, P=0.046), and mRNA expression 
      levels of MMP-7 (r=0.511, P=0.006), TIMP-1 (r=0.320, P=0.040), TIMP-2 (r=0.428, 
      P=0.026), and TIMP-3 (r=0.553, P=0.003). This study suggests that expression of 
      MMPs is associated with an inflammatory reaction in the early phase and TIMPs 
      expression at the late phase may contribute to both fibrosis and liver injury. 
      MMPs and TIMPs may serve as diagnostic markers for the severity of O. 
      viverrini-induced liver injury.
FAU - Prakobwong, Suksanti
AU  - Prakobwong S
AD  - Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 
      Thailand.
FAU - Pinlaor, Somchai
AU  - Pinlaor S
FAU - Yongvanit, Puangrat
AU  - Yongvanit P
FAU - Sithithaworn, Paiboon
AU  - Sithithaworn P
FAU - Pairojkul, Chawalit
AU  - Pairojkul C
FAU - Hiraku, Yusuke
AU  - Hiraku Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090103
PL  - England
TA  - Int J Parasitol
JT  - International journal for parasitology
JID - 0314024
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - RMB44WO89X (Hydroxyproline)
SB  - IM
MH  - Animals
MH  - Asia, Southeastern
MH  - Bile Duct Diseases/*enzymology/pathology
MH  - Collagen/*metabolism
MH  - Cricetinae
MH  - Cytokines/metabolism
MH  - Electrophoresis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fibrosis/enzymology/*pathology
MH  - Gene Expression
MH  - Gene Expression Profiling
MH  - Humans
MH  - Hydroxyproline/metabolism
MH  - Immunohistochemistry
MH  - Liver/chemistry/metabolism/pathology
MH  - Liver Cirrhosis/enzymology/pathology
MH  - Male
MH  - Matrix Metalloproteinases/genetics/*metabolism
MH  - Opisthorchiasis/*metabolism/pathology
MH  - Opisthorchis/parasitology
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
MH  - Tissue Inhibitor of Metalloproteinases/genetics/*metabolism
EDAT- 2009/01/27 09:00
MHDA- 2010/04/17 06:00
CRDT- 2009/01/27 09:00
PHST- 2008/08/28 00:00 [received]
PHST- 2008/11/04 00:00 [revised]
PHST- 2008/12/05 00:00 [accepted]
PHST- 2009/01/27 09:00 [entrez]
PHST- 2009/01/27 09:00 [pubmed]
PHST- 2010/04/17 06:00 [medline]
AID - S0020-7519(08)00484-0 [pii]
AID - 10.1016/j.ijpara.2008.12.002 [doi]
PST - ppublish
SO  - Int J Parasitol. 2009 Jun;39(7):825-35. doi: 10.1016/j.ijpara.2008.12.002. Epub 
      2009 Jan 3.