PMID- 19168629
OWN - NLM
STAT- MEDLINE
DCOM- 20090227
LR  - 20211020
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 106
IP  - 5
DP  - 2009 Feb 3
TI  - Antibody to Langerin/CD207 localizes large numbers of CD8alpha+ dendritic cells 
      to the marginal zone of mouse spleen.
PG  - 1524-9
LID - 10.1073/pnas.0812247106 [doi]
AB  - Dendritic cells (DCs) are strategically positioned to take up antigens and 
      initiate adaptive immunity. One DC subset expresses CD8alphaalpha in mice and is 
      specialized to capture dying cells and process antigens for MHC class I 
      "cross-presentation." Because CD8(+) DCs also express DEC205/CD205, which is 
      localized to splenic T cell regions, it is thought that CD8(+) DCs also are 
      restricted to T zones. Here, we used a new antibody to Langerin/CD207, which 
      colabels isolated CD8(+) CD205(+) DCs, to immunolabel spleen sections. The mAb 
      labeled discrete cells with high levels of CD11c and CD8. Surprisingly most 
      CD207(+) profiles were in marginal zones surrounding splenic white pulp nodules, 
      and only smaller numbers were in T cell areas, where CD205 colabeling was noted. 
      Despite a marginal zone location, CD207(+) DCs lacked identifying molecules for 3 
      different types of macrophages, localized in proximity and, in contrast to 
      macrophages, marginal zone DCs were poor scavengers of soluble and particulate 
      substrates. After stimulation with microbial agonists, Langerin expression 
      disappeared from the marginal zone at 6-12 h, but was greatly expanded in the T 
      cell areas, and by 24-48 h, Langerin expression disappeared. Therefore, 
      anti-Langerin antibodies localize a majority of CD8(+) DCs to non-T cell regions 
      of mouse spleen, where they are distinct from adjacent macrophages.
FAU - Idoyaga, Juliana
AU  - Idoyaga J
AD  - Laboratory of Cellular Physiology and Immunology and Chris Browne Center for 
      Immunology and Immune Diseases, The Rockefeller University, 1230 York Avenue, New 
      York, NY 10065, USA.
FAU - Suda, Nao
AU  - Suda N
FAU - Suda, Koji
AU  - Suda K
FAU - Park, Chae Gyu
AU  - Park CG
FAU - Steinman, Ralph M
AU  - Steinman RM
LA  - eng
GR  - P01 AI051573/AI/NIAID NIH HHS/United States
GR  - R01 AI013013/AI/NIAID NIH HHS/United States
GR  - AI13013/AI/NIAID NIH HHS/United States
GR  - AI51573/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090123
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Surface)
RN  - 0 (Cd207 protein, mouse)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Mannose-Binding Lectins)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Antigens, Surface/*immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Flow Cytometry
MH  - Lectins, C-Type/*immunology
MH  - Macrophages/immunology
MH  - Mannose-Binding Lectins/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Microscopy, Fluorescence
MH  - Phagocytosis
MH  - Spleen/*immunology
PMC - PMC2635812
COIS- The authors declare no conflict of interest.
EDAT- 2009/01/27 09:00
MHDA- 2009/02/28 09:00
PMCR- 2009/08/03
CRDT- 2009/01/27 09:00
PHST- 2009/01/27 09:00 [entrez]
PHST- 2009/01/27 09:00 [pubmed]
PHST- 2009/02/28 09:00 [medline]
PHST- 2009/08/03 00:00 [pmc-release]
AID - 0812247106 [pii]
AID - 6436 [pii]
AID - 10.1073/pnas.0812247106 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2009 Feb 3;106(5):1524-9. doi: 10.1073/pnas.0812247106. 
      Epub 2009 Jan 23.