PMID- 19169706
OWN - NLM
STAT- MEDLINE
DCOM- 20090818
LR  - 20211020
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 454
IP  - 3
DP  - 2009 Mar
TI  - Comprehensive immunohistochemical analysis of Her-2/neu oncoprotein 
      overexpression in breast cancer: HercepTest (Dako) for manual testing and 
      Her-2/neuTest 4B5 (Ventana) for Ventana BenchMark automatic staining system with 
      correlation to results of fluorescence in situ hybridization (FISH).
PG  - 241-8
LID - 10.1007/s00428-009-0728-8 [doi]
AB  - Overexpression of Her-2/neu-oncoprotein is used as marker for Herceptin therapy. 
      To investigate the sensitivity and specificity of automatic immunohistochemistry 
      (Benchmark, Ventana), we compared the results to the manual testing (Dako) in 130 
      breast carcinomas and validated the results by fluorescence in situ hybridization 
      (FISH). Manual and automatic immunohistochemistry of Her-2/neu-oncoprotein using 
      two different antibodies (HercepTest, Her-2/neuTest 4B5) was analyzed. FISH was 
      performed in all cases with uncertain or strong overexpression in either 
      immunohistochemical stainings or with different immunohistochemical results. Same 
      immunohistochemical results were seen in 73.8%. Two cases with overexpression, 
      detected with Her-2/neuTest 4B5 and confirmed by FISH, showed no overexpression 
      using HercepTest. From 21 cases with 2+ by Her-2/neuTest 4B5, 15 cases had no 
      gene amplification (two of them with 3+ HercepTest); three cases showed a gene 
      amplification (one of them with failing overexpression by HercepTest); two other 
      cases were polysomic; one could not be analyzed. Ventana immunohistochemistry 
      seems to be of same reliability like Dako with a little better concordance to 
      FISH in our study.
FAU - Mayr, Doris
AU  - Mayr D
AD  - Pathologisches Institut, Ludwig Maximilians Universitaet Munchen, Thalkirchner 
      Strasse 36, 80337, Munchen, Germany. doris.mayr@med.uni-muenchen.de
FAU - Heim, Sibylle
AU  - Heim S
FAU - Werhan, Cedric
AU  - Werhan C
FAU - Zeindl-Eberhart, Evelyn
AU  - Zeindl-Eberhart E
FAU - Kirchner, Thomas
AU  - Kirchner T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20090124
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Reagent Kits, Diagnostic)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/genetics/*metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - Reagent Kits, Diagnostic
MH  - Receptor, ErbB-2/*analysis/biosynthesis/genetics
MH  - Sensitivity and Specificity
MH  - Staining and Labeling/methods
EDAT- 2009/01/27 09:00
MHDA- 2009/08/19 09:00
CRDT- 2009/01/27 09:00
PHST- 2008/07/31 00:00 [received]
PHST- 2009/01/05 00:00 [accepted]
PHST- 2008/12/23 00:00 [revised]
PHST- 2009/01/27 09:00 [entrez]
PHST- 2009/01/27 09:00 [pubmed]
PHST- 2009/08/19 09:00 [medline]
AID - 10.1007/s00428-009-0728-8 [doi]
PST - ppublish
SO  - Virchows Arch. 2009 Mar;454(3):241-8. doi: 10.1007/s00428-009-0728-8. Epub 2009 
      Jan 24.