PMID- 19170032
OWN - NLM
STAT- MEDLINE
DCOM- 20090304
LR  - 20161021
IS  - 1934-8258 (Electronic)
IS  - 1934-8258 (Linking)
VI  - Chapter 8
DP  - 2009 Jan
TI  - Preparation and culture of products of conception and other solid tissues for 
      chromosome analysis.
PG  - Unit 8.5
LID - 10.1002/0471142905.hg0805s60 [doi]
AB  - In clinical settings, chromosome studies are usually performed on solid tissue 
      other than solid tumors for one of two reasons: the tissue biopsy is the only 
      tissue available from the patient, or tissues other than the standard peripheral 
      blood lymphocytes must be examined because of suspected mosaicism. This unit 
      describes methods for culturing tissue samples to be used for preparation of 
      metaphase chromosomes or for biochemical or DNA analysis. Protocols include an 
      efficient enzymatic cell disruption procedure and a mechanical disruption 
      procedure. In addition, a procedure for processing uncultured cells for analysis 
      by fluorescence in situ hybridization (FISH) is provided. Finally, a method is 
      presented that can sometimes be applied to placental tissue involving the use of 
      mitotically active chorionic villus tissue to prepare metaphase chromosome 
      spreads directly, without cell culture. Two support protocols describe methods 
      for collecting tissue samples.
FAU - Levy, Brynn
AU  - Levy B
AD  - College of Physicians and Surgeons of Columbia University, New York, USA.
FAU - Jobanputra, Vaidehi
AU  - Jobanputra V
FAU - Warburton, Dorothy
AU  - Warburton D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Curr Protoc Hum Genet
JT  - Current protocols in human genetics
JID - 101287858
SB  - IM
MH  - Biopsy/*methods
MH  - Cytogenetic Analysis/*methods
MH  - Embryo, Mammalian/*cytology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Placenta/*cytology
MH  - Tissue Culture Techniques/*methods
EDAT- 2009/01/27 09:00
MHDA- 2009/03/05 09:00
CRDT- 2009/01/27 09:00
PHST- 2009/01/27 09:00 [entrez]
PHST- 2009/01/27 09:00 [pubmed]
PHST- 2009/03/05 09:00 [medline]
AID - 10.1002/0471142905.hg0805s60 [doi]
PST - ppublish
SO  - Curr Protoc Hum Genet. 2009 Jan;Chapter 8:Unit 8.5. doi: 
      10.1002/0471142905.hg0805s60.