PMID- 19173059
OWN - NLM
STAT- MEDLINE
DCOM- 20091009
LR  - 20090128
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 134
IP  - 2
DP  - 2009 Feb
TI  - Artificial-enzyme gel membrane-based biosurveillance sensor with high 
      reproducibility and long-term storage stability.
PG  - 337-42
LID - 10.1039/b810846c [doi]
AB  - We propose that the most sophisticated strategy for primary biosurveillance is to 
      exploit structural commonality through the detection of biologically relevant 
      phosphoric substances. A novel assay, an artificial-enzyme membrane was designed 
      and synthesized for sensor fabrication. This artificial-enzyme catalyzes the 
      hydrolysis of the diphosphoric acid anhydride structure. This 
      structure-selective, albeit not molecule-selective, catalytic hydrolysis was 
      successfully coupled with amperometric detection. Since the catalytic reaction 
      produces a dephosphorylation product (PO(4)(3-)), it can be reduced by an 
      electrode potential of -250 mV vs. Ag/AgCl. Owing to the structural selectivity 
      of the artificial-enzyme membrane, the sensor can detect biological phosphoric 
      substances comprehensively that have the diphosphoric acid anhydride structure. 
      The sensor successfully determined various biological phosphoric substances at 
      concentrations in the micromolar (microM) to millimolar (mM) range, and it showed 
      good functional stability and reproducibility in terms of sensor responses. This 
      sensor was used to detect Escherichia coli lysed by heat treatment, and the 
      response increased with increasing bacterial numbers. This unique technique for 
      analyzing molecular commonality can be applied to the surveillance of 
      biocontaminants, e.g. microorganisms, spores and viruses. Artificial-enzyme-based 
      detection is a novel strategy for practical biosurveillance in the front line.
FAU - Ikeno, Shinya
AU  - Ikeno S
AD  - Department of Biological functions and Engineering, Kyushu Institute of 
      Technology, Kitakyushu Science and Research Park, Fukuoka, 808-0196, Japan.
FAU - Yoshida, Tetsuya
AU  - Yoshida T
FAU - Haruyama, Tetsuya
AU  - Haruyama T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081118
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Environmental Pollutants)
RN  - 0 (Gels)
RN  - 0 (Membranes, Artificial)
RN  - 0 (Phosphates)
SB  - IM
MH  - *Biosensing Techniques
MH  - Biosurveillance/*methods
MH  - Environmental Pollutants/*analysis
MH  - Equipment Design
MH  - Escherichia coli/isolation & purification
MH  - Gels
MH  - Membranes, Artificial
MH  - Phosphates/analysis
MH  - Viruses/isolation & purification
EDAT- 2009/01/29 09:00
MHDA- 2009/10/10 06:00
CRDT- 2009/01/29 09:00
PHST- 2009/01/29 09:00 [entrez]
PHST- 2009/01/29 09:00 [pubmed]
PHST- 2009/10/10 06:00 [medline]
AID - 10.1039/b810846c [doi]
PST - ppublish
SO  - Analyst. 2009 Feb;134(2):337-42. doi: 10.1039/b810846c. Epub 2008 Nov 18.