PMID- 19175933
OWN - NLM
STAT- MEDLINE
DCOM- 20090317
LR  - 20211020
IS  - 1471-213X (Electronic)
IS  - 1471-213X (Linking)
VI  - 9
DP  - 2009 Jan 28
TI  - Analyses of zebrafish and Xenopus oocyte maturation reveal conserved and diverged 
      features of translational regulation of maternal cyclin B1 mRNA.
PG  - 7
LID - 10.1186/1471-213X-9-7 [doi]
AB  - BACKGROUND: Vertebrate development relies on the regulated translation of stored 
      maternal mRNAs, but how these regulatory mechanisms may have evolved to control 
      translational efficiency of individual mRNAs is poorly understood. We compared 
      the translational regulation and polyadenylation of the cyclin B1 mRNA during 
      zebrafish and Xenopus oocyte maturation. Polyadenylation and translational 
      activation of cyclin B1 mRNA is well characterized during Xenopus oocyte 
      maturation. Specifically, Xenopus cyclin B1 mRNA is polyadenylated and 
      translationally activated during oocyte maturation by proteins that recognize the 
      conserved AAUAAA hexanucleotide and U-rich Cytoplasmic Polyadenylation Elements 
      (CPEs) within cyclin B1 mRNA's 3'UnTranslated Region (3'UTR). RESULTS: The 
      zebrafish cyclin B1 mRNA was polyadenylated during zebrafish oocyte maturation. 
      Furthermore, the zebrafish cyclin B1 mRNA's 3'UTR was sufficient to stimulate 
      translation of a reporter mRNA during zebrafish oocyte maturation. This 
      stimulation required both AAUAAA and U-rich CPE-like sequences. However, in 
      contrast to AAUAAA, the positions and sequences of the functionally defined CPEs 
      were poorly conserved between Xenopus and zebrafish cyclin B1 mRNA 3'UTRs. To 
      determine whether these differences were relevant to translation efficiency, we 
      analyzed the translational activity of reporter mRNAs containing either the 
      zebrafish or Xenopus cyclin B1 mRNA 3'UTRs during both zebrafish and Xenopus 
      oocyte maturation. The zebrafish cyclin B1 3'UTR was quantitatively less 
      effective at stimulating polyadenylation and translation compared to the Xenopus 
      cyclin B1 3'UTR during both zebrafish and Xenopus oocyte maturation. CONCLUSION: 
      Although the factors that regulate translation of maternal mRNAs are highly 
      conserved, the target sequences and overall sequence architecture within the 
      3'UTR of the cyclin B1 mRNA have diverged to affect translational efficiency, 
      perhaps to optimize levels of cyclin B1 protein required by these different 
      species during their earliest embryonic cell divisions.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - University of Wisconsin School of Medicine and Public Health, Department of 
      Biomolecular Chemistry, 1300 University Avenue Madison, Madison, Wisconsin 53706, 
      USA. zhang7@wisc.edu
FAU - Sheets, Michael D
AU  - Sheets MD
LA  - eng
GR  - R01 HD043996/HD/NICHD NIH HHS/United States
GR  - HD43996/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090128
PL  - England
TA  - BMC Dev Biol
JT  - BMC developmental biology
JID - 100966973
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Cyclin B)
RN  - 0 (Cyclin B1)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - Animals
MH  - Cyclin B/*genetics
MH  - Cyclin B1
MH  - Female
MH  - *Gene Expression Regulation, Developmental/genetics/physiology
MH  - Oocytes/*metabolism
MH  - Polyadenylation/genetics/physiology
MH  - Protein Biosynthesis/genetics/physiology
MH  - RNA, Messenger/*genetics
MH  - Xenopus
MH  - Zebrafish
PMC - PMC2644680
EDAT- 2009/01/30 09:00
MHDA- 2009/03/18 09:00
PMCR- 2009/01/28
CRDT- 2009/01/30 09:00
PHST- 2008/06/19 00:00 [received]
PHST- 2009/01/28 00:00 [accepted]
PHST- 2009/01/30 09:00 [entrez]
PHST- 2009/01/30 09:00 [pubmed]
PHST- 2009/03/18 09:00 [medline]
PHST- 2009/01/28 00:00 [pmc-release]
AID - 1471-213X-9-7 [pii]
AID - 10.1186/1471-213X-9-7 [doi]
PST - epublish
SO  - BMC Dev Biol. 2009 Jan 28;9:7. doi: 10.1186/1471-213X-9-7.