PMID- 19176056
OWN - NLM
STAT- MEDLINE
DCOM- 20100527
LR  - 20090129
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 29
IP  - 10
DP  - 2008 Oct
TI  - [Role of multiple myeloma cells on normal endothelial cells in co-culture 
      system].
PG  - 658-61
AB  - OBJECTIVE: To study the influence of multiple myeloma (MM) cells on normal 
      endothelial cells in co-culture system in vitro. METHODS: A co-culture system of 
      human MM cell line RPMI8226 with human umbilical vein endothelial cells (HUVECs) 
      was established in vitro. Mono-cultured normal endothelial cells were used as 
      control. Light microscopy and transmission electron microscopy were used to 
      observe the morphology of the endothelial cells. The effects of HUVECs 
      co-cultured with RPMI8226 on HUVECs angiogenesis were studied by modified 
      transwell migration assay and net-like formation assay. The protein expression of 
      brain derived neurotrophic factor (BDNF), TrkB, Endoglin, Tie-2, beta 3 integrin 
      and vascular cell adhesion molecule-1 (VCAM-1) in HUVECs were determined by FACS 
      and Western blot analysis, respectively. RESULTS: The morphology of HUVECs 
      co-cultured with RPMI8226 cells became a narrower apart of extended shape as they 
      began to align themselves. The sizes of nucleus and nucleolus were enlarged with 
      an increased ratio of nuclear to nucleoplasm. The endoplasm was lose and 
      distorted and the number of surface microvilli decreased. The RPMI8226 cell 
      stimulated the migration and net-like formation of HUVEC, the number of net-like 
      structure and migration cell being increased by 112% and 136%, respectively, 
      compared with that of mono-cultured HUVECs. The expressions of BDNF, TrkB, 
      Endoglin, Tie-2, beta 3 integrin and VCAM-1 in the ECs co-cultured with RPMI8226 
      were all up-regulated in comparison with those in the controls. CONCLUSION: The 
      MM cells promote formation of new vessels in co-cultured endothelial cells and 
      the endothelial cells in MM are different from the normal ECs in character, and 
      behavior.
FAU - Wang, Ya-Dan
AU  - Wang YD
AD  - Institution of Hematology, Union Hospital Affiliated to Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430022, China.
FAU - Hu, Yu
AU  - Hu Y
FAU - Sun, Chun-Yan
AU  - Sun CY
FAU - Wang, Hua-Fang
AU  - Wang HF
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Endothelial Cells/*cytology
MH  - Endothelium, Vascular/cytology
MH  - Humans
MH  - Multiple Myeloma/metabolism/*pathology
MH  - Neovascularization, Physiologic
MH  - Umbilical Veins/cytology
MH  - Vascular Cell Adhesion Molecule-1/metabolism
EDAT- 2009/01/30 09:00
MHDA- 2010/05/28 06:00
CRDT- 2009/01/30 09:00
PHST- 2009/01/30 09:00 [entrez]
PHST- 2009/01/30 09:00 [pubmed]
PHST- 2010/05/28 06:00 [medline]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2008 Oct;29(10):658-61.