PMID- 1917816
OWN - NLM
STAT- MEDLINE
DCOM- 19911031
LR  - 20151119
IS  - 0004-5756 (Print)
IS  - 0004-5756 (Linking)
VI  - 74
IP  - 4
DP  - 1991 Jul-Aug
TI  - Modified gas chromatographic/mass spectrometric method for determination of 
      daminozide in high protein food products.
PG  - 682-92
AB  - A modified version of the Conditt and Baumgardner gas chromatographic/mass 
      spectroscopic (GC/MS) method for determination of daminozide in peanut butter and 
      raw peanuts is described. Daminozide in the food product is hydrolyzed to 
      unsymmetrical dimethylhydrazine (UDMH) by sodium hydroxide digestion. The 
      generated UDMH is distilled from the food matrix and captured by reaction with 
      salicylaldehyde in a condensation trap. Resulting high pH distillates generated 
      by peanuts and peanut products are adjusted back to a pH of 5-6 through addition 
      of glacial acetic acid. After thermal incubation and extraction into methylene 
      chloride, salicylaldehyde dimethylhydrazone is separated from interferences by 
      capillary GC and quantitated by MS using the selective ion monitoring (SIM) mode. 
      Quantitation of daminozide is based on the ratio of the salicylaldehyde 
      dimethylhydrazone molecular ion (m/z 164) to the molecular ion (m/z 153) of the 
      internal standard, 4-nitroanisole. Confirmation of daminozide identity is 
      determined by relative intensity of the m/z 164 ion to the m/z 120 (C7H4ON) ion. 
      Improved m/z 164 ion intensity and reduction of neighboring interferences due to 
      acetic acid treatment permitted a daminozide detection limit of 0.005 ppm in a 50 
      g sample and an associated 0.02 ppm limit of quantitation. This modification is 
      specific for high protein samples that generate high pH distillates such as 
      peanuts and peanut products and is not specifically intended for analysis of low 
      protein samples.
FAU - Faughnan, K T
AU  - Faughnan KT
AD  - Best Foods Research and Engineering Center, Analytical Research Laboratory, 
      Union, NJ 07083.
FAU - Woodruff, M A
AU  - Woodruff MA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Assoc Off Anal Chem
JT  - Journal - Association of Official Analytical Chemists
JID - 7505559
RN  - 0 (Dietary Proteins)
RN  - 0 (Herbicides)
RN  - 0 (Proteins)
RN  - 0 (Succinates)
RN  - F6KF33M5UB (daminozide)
SB  - IM
MH  - Arachis/chemistry
MH  - Dietary Proteins/analysis
MH  - Food Analysis/*methods
MH  - Gas Chromatography-Mass Spectrometry/methods
MH  - Herbicides/*analysis
MH  - Hydrogen-Ion Concentration
MH  - Microchemistry/methods
MH  - Proteins/analysis
MH  - Succinates/*analysis
EDAT- 1991/07/01 00:00
MHDA- 1991/07/01 00:01
CRDT- 1991/07/01 00:00
PHST- 1991/07/01 00:00 [pubmed]
PHST- 1991/07/01 00:01 [medline]
PHST- 1991/07/01 00:00 [entrez]
PST - ppublish
SO  - J Assoc Off Anal Chem. 1991 Jul-Aug;74(4):682-92.