PMID- 19178404
OWN - NLM
STAT- MEDLINE
DCOM- 20090406
LR  - 20090130
IS  - 1398-9995 (Electronic)
IS  - 0105-4538 (Linking)
VI  - 64
IP  - 2
DP  - 2009 Feb
TI  - Negativization rates of IgE radioimmunoassay and basophil activation test in 
      immediate reactions to penicillins.
PG  - 242-8
LID - 10.1111/j.1398-9995.2008.01713.x [doi]
AB  - BACKGROUND: Skin test sensitivity in patients with immediate allergy to 
      penicillins tends to decrease over time, but no information is available 
      concerning in vitro tests. We analysed the negativization rates of two in vitro 
      methods that determine specific immunoglobulin E (IgE) antibodies, the basophil 
      activation test using flow cytometry (BAT) and the radioallergosorbent test 
      (RAST), in immediate allergic reactions to penicillins. METHODS: Forty-one 
      patients with immediate allergic reactions to amoxicillin were followed up over a 
      4-year period. BAT and RAST were performed at 6-month intervals. Patients were 
      randomized into groups: Group I, skin tests carried out at regular intervals; 
      Group II, skin tests made only at the beginning of the study. RESULTS: 
      Differences were observed between RAST and BAT (P < 0.01), the latter showing 
      earlier negativization. Considering different haptens, significant differences 
      for the rate of negativization were only found for amoxicillin (P < 0.05). 
      Comparisons between Groups I (n = 10) and II (n = 31) showed a tendency to become 
      negative later in Group I with RAST. CONCLUSIONS: Levels of specific IgE 
      antibodies tended to decrease over time in patients with immediate allergic 
      reactions to amoxicillin. Conversion to negative took longer for the RAST assay, 
      although the differences were only detected with the amoxicillin hapten. Skin 
      testing influenced the rate of negativization of the RAST assay, contributing to 
      maintenance of in vitro sensitivity. Because of the loss of sensitivity over 
      time, the determination of specific IgE antibodies to penicillins in patients 
      with immediate allergic reactions must be done as soon as possible after the 
      reaction.
FAU - Fernandez, T D
AU  - Fernandez TD
AD  - Research Unit for Allergic Diseases, Carlos Haya Hospital, Malaga, Spain.
FAU - Torres, M J
AU  - Torres MJ
FAU - Blanca-Lopez, N
AU  - Blanca-Lopez N
FAU - Rodriguez-Bada, J L
AU  - Rodriguez-Bada JL
FAU - Gomez, E
AU  - Gomez E
FAU - Canto, G
AU  - Canto G
FAU - Mayorga, C
AU  - Mayorga C
FAU - Blanca, M
AU  - Blanca M
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Penicillins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Anti-Bacterial Agents/adverse effects/*immunology
MH  - Basophil Degranulation Test
MH  - Basophils/*immunology/metabolism
MH  - Drug Hypersensitivity/*diagnosis/immunology
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Hypersensitivity, Immediate/*diagnosis/immunology
MH  - Immunoglobulin E/*blood
MH  - Male
MH  - Middle Aged
MH  - Penicillins/adverse effects/*immunology
MH  - Radioallergosorbent Test
EDAT- 2009/01/31 09:00
MHDA- 2009/04/07 09:00
CRDT- 2009/01/31 09:00
PHST- 2009/01/31 09:00 [entrez]
PHST- 2009/01/31 09:00 [pubmed]
PHST- 2009/04/07 09:00 [medline]
AID - ALL1713 [pii]
AID - 10.1111/j.1398-9995.2008.01713.x [doi]
PST - ppublish
SO  - Allergy. 2009 Feb;64(2):242-8. doi: 10.1111/j.1398-9995.2008.01713.x.