PMID- 19181454 OWN - NLM STAT- MEDLINE DCOM- 20090825 LR - 20211020 IS - 1873-3360 (Electronic) IS - 0306-4530 (Print) IS - 0306-4530 (Linking) VI - 34 IP - 6 DP - 2009 Jul TI - Chronic stress increases pituitary adenylate cyclase-activating peptide (PACAP) and brain-derived neurotrophic factor (BDNF) mRNA expression in the bed nucleus of the stria terminalis (BNST): roles for PACAP in anxiety-like behavior. PG - 833-43 LID - 10.1016/j.psyneuen.2008.12.013 [doi] AB - Exposure to chronic stress has been argued to produce maladaptive anxiety-like behavioral states, and many of the brain regions associated with stressor responding also mediate anxiety-like behavior. Pituitary adenylate cyclase activating polypeptide (PACAP) and its specific G protein-coupled PAC(1) receptor have been associated with many of these stress- and anxiety-associated brain regions, and signaling via this peptidergic system may facilitate the neuroplasticity associated with pathological affective states. Here we investigated whether chronic stress increased transcript expression for PACAP, PAC(1) receptor, brain-derived neurotrophic factor (BDNF), and tyrosine receptor kinase B (TrkB) in several nuclei. In rats exposed to a 7 days chronic variate stress paradigm, chronic stress enhanced baseline startle responding induced by handling and exposure to bright lights. Following chronic stress, quantitative transcript assessments of brain regions demonstrated dramatic increases in PACAP and PAC(1) receptor, BDNF, and TrkB receptor mRNA expression selectively in the dorsal aspect of the anterolateral bed nucleus of the stria terminalis (dBNST). Related vasoactive intestinal peptide (VIP) and VPAC receptor, and other stress peptide transcript levels were not altered compared to controls. Moreover, acute PACAP38 infusion into the dBNST resulted in a robust dose-dependent anxiogenic response on baseline startle responding that persisted for 7 days. PACAP/PAC(1) receptor signaling has established trophic functions and its coordinate effects with chronic stress-induced dBNST BDNF and TrkB transcript expression may underlie the maladaptive BNST remodeling and plasticity associated with anxiety-like behavior. FAU - Hammack, Sayamwong E AU - Hammack SE AD - Department of Psychology, University of Vermont, Burlington, VT 05405, USA. shammack@uvm.edu FAU - Cheung, Joseph AU - Cheung J FAU - Rhodes, Kimberly M AU - Rhodes KM FAU - Schutz, Kristin C AU - Schutz KC FAU - Falls, William A AU - Falls WA FAU - Braas, Karen M AU - Braas KM FAU - May, Victor AU - May V LA - eng GR - R01 HD027468/HD/NICHD NIH HHS/United States GR - P20 RR016435/RR/NCRR NIH HHS/United States GR - HD27468/HD/NICHD NIH HHS/United States GR - P20RR16435/RR/NCRR NIH HHS/United States GR - R01 NS037179-01A2/NS/NINDS NIH HHS/United States GR - R01 HD027468-13/HD/NICHD NIH HHS/United States GR - NS37179/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20090131 PL - England TA - Psychoneuroendocrinology JT - Psychoneuroendocrinology JID - 7612148 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Pituitary Adenylate Cyclase-Activating Polypeptide) RN - 0 (RNA, Messenger) RN - 0 (Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Adaptation, Psychological/drug effects MH - Animals MH - Anxiety/chemically induced/*etiology/genetics/metabolism MH - Behavior, Animal/drug effects/physiology MH - Brain-Derived Neurotrophic Factor/*genetics/metabolism MH - Chronic Disease MH - Male MH - Models, Biological MH - Neuronal Plasticity/drug effects/genetics MH - Pituitary Adenylate Cyclase-Activating Polypeptide/*genetics/metabolism/pharmacology/*physiology MH - RNA, Messenger/metabolism MH - Rats MH - Rats, Sprague-Dawley MH - Receptor, trkB/genetics/metabolism MH - Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I/genetics/metabolism MH - Septal Nuclei/drug effects/*metabolism MH - Stress, Psychological/chemically induced/*genetics/metabolism MH - Time Factors MH - Up-Regulation/drug effects/genetics PMC - PMC2705919 MID - NIHMS118804 EDAT- 2009/02/03 09:00 MHDA- 2009/08/26 09:00 PMCR- 2010/07/01 CRDT- 2009/02/03 09:00 PHST- 2008/09/22 00:00 [received] PHST- 2008/11/26 00:00 [revised] PHST- 2008/12/18 00:00 [accepted] PHST- 2009/02/03 09:00 [entrez] PHST- 2009/02/03 09:00 [pubmed] PHST- 2009/08/26 09:00 [medline] PHST- 2010/07/01 00:00 [pmc-release] AID - S0306-4530(08)00339-9 [pii] AID - 10.1016/j.psyneuen.2008.12.013 [doi] PST - ppublish SO - Psychoneuroendocrinology. 2009 Jul;34(6):833-43. doi: 10.1016/j.psyneuen.2008.12.013. Epub 2009 Jan 31.