PMID- 19183140
OWN - NLM
STAT- MEDLINE
DCOM- 20090707
LR  - 20151119
IS  - 1530-0277 (Electronic)
IS  - 0145-6008 (Linking)
VI  - 33
IP  - 4
DP  - 2009 Apr
TI  - Dietary zinc supplementation throughout pregnancy protects against fetal 
      dysmorphology and improves postnatal survival after prenatal ethanol exposure in 
      mice.
PG  - 591-600
LID - 10.1111/j.1530-0277.2008.00873.x [doi]
AB  - BACKGROUND: We have previously demonstrated that ethanol teratogenicity is 
      associated with metallothionein-induced fetal zinc (Zn) deficiency, and that 
      maternal subcutaneous Zn treatment given with ethanol in early pregnancy prevents 
      fetal abnormalities and spatial memory impairments in mice. Here we investigated 
      whether dietary Zn supplementation throughout pregnancy can also prevent 
      ethanol-related dysmorphology. METHODS: Pregnant mice were injected with saline 
      or 25% ethanol (0.015 ml/g intraperitoneally at 0 and 4 hours) on gestational day 
      (GD) 8 and fed either a control (35 mg Zn/kg) or a Zn-supplemented diet (200 mg 
      Zn/kg) from GD 0 to 18. Fetuses from the saline, saline + Zn, ethanol and ethanol 
      + Zn groups were assessed for external birth abnormalities on GD 18. In a 
      separate cohort of mice, postnatal growth and survival of offspring from these 
      treatment groups were examined from birth until postnatal day 60. RESULTS: 
      Fetuses from dams treated with ethanol alone in early pregnancy had a 
      significantly greater incidence of physical abnormalities (26%) compared to those 
      from the saline (10%), saline + Zn (9%), or ethanol + Zn (12%) groups. The 
      incidence of abnormalities in ethanol + Zn-supplemented fetuses was not different 
      from saline-treated fetuses. While ethanol exposure did not affect the number of 
      fetal resorptions or pre- or postnatal weight, there were more stillbirths with 
      ethanol alone, and cumulative postnatal mortality was significantly higher in 
      offspring exposed to ethanol alone (35% deaths) compared to all other treatment 
      groups (13.5 to 20.5% deaths). Mice supplemented with Zn throughout pregnancy had 
      higher plasma Zn concentrations than those in un-supplemented groups. 
      CONCLUSIONS: These findings demonstrate that dietary Zn supplementation 
      throughout pregnancy ameliorates dysmorphology and postnatal mortality caused by 
      ethanol exposure in early pregnancy.
FAU - Summers, Brooke L
AU  - Summers BL
AD  - Hanson Institute/Institute of Medical and Veterinary Science, Adelaide, SA, 
      Australia.
FAU - Rofe, Allan M
AU  - Rofe AM
FAU - Coyle, Peter
AU  - Coyle P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090112
PL  - England
TA  - Alcohol Clin Exp Res
JT  - Alcoholism, clinical and experimental research
JID - 7707242
RN  - 0 (Central Nervous System Depressants)
RN  - 3K9958V90M (Ethanol)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 9038-94-2 (Metallothionein)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Abnormalities, Drug-Induced/*prevention & control
MH  - Alcoholism/complications
MH  - Animals
MH  - Central Nervous System Depressants/*adverse effects/pharmacology
MH  - Dietary Supplements
MH  - Disease Models, Animal
MH  - Embryonic Development/*drug effects
MH  - Ethanol/*adverse effects/pharmacology
MH  - Female
MH  - Liver/metabolism
MH  - Male
MH  - Metallothionein/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - Pregnancy, Animal/*drug effects
MH  - Prenatal Exposure Delayed Effects/chemically induced/*prevention & control
MH  - Sodium Chloride/pharmacology
MH  - Zinc/administration & dosage/metabolism/*pharmacology
EDAT- 2009/02/03 09:00
MHDA- 2009/07/08 09:00
CRDT- 2009/02/03 09:00
PHST- 2009/02/03 09:00 [entrez]
PHST- 2009/02/03 09:00 [pubmed]
PHST- 2009/07/08 09:00 [medline]
AID - ACER873 [pii]
AID - 10.1111/j.1530-0277.2008.00873.x [doi]
PST - ppublish
SO  - Alcohol Clin Exp Res. 2009 Apr;33(4):591-600. doi: 
      10.1111/j.1530-0277.2008.00873.x. Epub 2009 Jan 12.