PMID- 19183259
OWN - NLM
STAT- MEDLINE
DCOM- 20090402
LR  - 20131121
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Linking)
VI  - 108
IP  - 6
DP  - 2009 Mar
TI  - Lithium enhances the neuronal differentiation of neural progenitor cells in vitro 
      and after transplantation into the avulsed ventral horn of adult rats through the 
      secretion of brain-derived neurotrophic factor.
PG  - 1385-98
LID - 10.1111/j.1471-4159.2009.05902.x [doi]
AB  - This study was undertaken to elucidate the molecular mechanisms by which lithium 
      regulates the development of spinal cord-derived neural progenitor cells (NPCs) 
      in vitro and after transplanted in vivo. Our results show that lithium at the 
      therapeutic concentration significantly increases the proliferation and neuronal 
      differentiation of NPCs in vitro. Specific ELISAs, western blotting, and 
      quantitative real-time RT-PCR assays demonstrate that lithium treatment 
      significantly elevates the expression and production of brain-derived 
      neurotrophic factor (BDNF) by NPCs in culture. Application of a BDNF neutralizing 
      antibody in culture leads to a marked reduction in the neurogenesis of 
      lithium-treated NPCs to the control level. However, it shows no effects on the 
      proliferation of lithium-treated NPCs. These findings suggest that the BDNF 
      pathway is possibly involved in the supportive role of lithium in inducing NPC 
      neurogenesis but not proliferation. This study also provides evidence that 
      lithium is able to elevate the neuronal generation and BDNF production of NPCs 
      after transplantation into the adult rat ventral horn with motoneuron 
      degeneration because of spinal root avulsion, which highlights the therapeutic 
      potential of lithium in cell replacement strategies for spinal cord injury 
      because of its ability to promote neuronal differentiation and BDNF production of 
      grafted NPCs in the injured spinal cord.
FAU - Su, Huanxing
AU  - Su H
AD  - Department of Anatomy, Development and Growth, Li Ka Shing Faculty of Medicine, 
      The University of Hong Kong, Pokfulam, Hong Kong SAR, China.
FAU - Zhang, Wenming
AU  - Zhang W
FAU - Guo, Jiasong
AU  - Guo J
FAU - Guo, Anchen
AU  - Guo A
FAU - Yuan, Qiuju
AU  - Yuan Q
FAU - Wu, Wutian
AU  - Wu W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090122
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Antibodies)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - G34N38R2N1 (Bromodeoxyuridine)
RN  - G4962QA067 (Lithium Chloride)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Animals, Newborn
MH  - Anterior Horn Cells/cytology
MH  - Antibodies/pharmacology
MH  - Brain-Derived Neurotrophic Factor/immunology/*metabolism
MH  - Bromodeoxyuridine/metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cell Transplantation/methods
MH  - Cells, Cultured
MH  - Embryo, Mammalian
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Female
MH  - Green Fluorescent Proteins/genetics
MH  - Lithium Chloride/*pharmacology
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurogenesis/*drug effects
MH  - Neurons/*drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Nerve Roots/cytology/metabolism/surgery
MH  - Stem Cells/*drug effects
EDAT- 2009/02/03 09:00
MHDA- 2009/04/03 09:00
CRDT- 2009/02/03 09:00
PHST- 2009/02/03 09:00 [entrez]
PHST- 2009/02/03 09:00 [pubmed]
PHST- 2009/04/03 09:00 [medline]
AID - JNC5902 [pii]
AID - 10.1111/j.1471-4159.2009.05902.x [doi]
PST - ppublish
SO  - J Neurochem. 2009 Mar;108(6):1385-98. doi: 10.1111/j.1471-4159.2009.05902.x. Epub 
      2009 Jan 22.