PMID- 19183966
OWN - NLM
STAT- MEDLINE
DCOM- 20090529
LR  - 20160512
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 393
IP  - 6-7
DP  - 2009 Mar
TI  - Analysing persistent organic pollutants in eggs, blood and tissue of the green 
      sea turtle (Chelonia mydas) using gas chromatography with tandem mass 
      spectrometry (GC-MS/MS).
PG  - 1719-31
LID - 10.1007/s00216-009-2608-0 [doi]
AB  - Investigation into persistent organic pollutants (POPs) in sea turtles is an 
      important area of conservation research due to the harmful effects of these 
      chemicals. However, the analysis of POPs in the green sea turtle (Chelonia mydas) 
      has been limited by methods with relatively high limits of detection and high 
      costs associated with multiple sample injections into complex arrangements of 
      analytical equipment. The present study aimed to develop a method that could 
      detect a large number of POPs in the blood, eggs and tissue of C. mydas at trace 
      concentrations. A gas chromatography with tandem mass spectrometry (GC-MS/MS) 
      method was developed that could report 125 POP compounds to a limit of detection 
      of <35 pg g(-1) using a single sample injection. The recoveries of internal 
      standards ranged from 30% to 96%, and the standard reference materials were 
      reported to within 70% of the certified values. The coefficient of variation of 
      ten replicates of pooled egg sample was <20% for all compounds, indicating low 
      within-run variation. This GC-MS/MS method is an improvement of previous methods 
      for analysing POPs in C. mydas in that more compounds can be reported at lower 
      concentrations and the accuracy and precision of the method are sound. This is 
      particularly important for C. mydas as they occupy a low trophic level and have 
      lower concentrations of POPs. This method is also simple to set up, and there are 
      minimal differences in sample preparation for the different tissue types.
FAU - van de Merwe, Jason Paul
AU  - van de Merwe JP
AD  - Griffith School of Environment and Australian Rivers Institute, Griffith 
      University, Gold Coast Campus, Gold Coast, 4222, QLD, Australia. 
      j.vandemerwe@griffith.edu.au
FAU - Hodge, Mary
AU  - Hodge M
FAU - Whittier, Joan Margaret
AU  - Whittier JM
FAU - Lee, Shing Yip
AU  - Lee SY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090131
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Organic Chemicals)
SB  - IM
MH  - Animals
MH  - Chromatography, Gas
MH  - Eggs/*analysis
MH  - Environmental Monitoring/methods
MH  - Muscle, Skeletal/*chemistry
MH  - Organic Chemicals/*analysis
MH  - Sensitivity and Specificity
MH  - Tandem Mass Spectrometry
MH  - Turtles
EDAT- 2009/02/03 09:00
MHDA- 2009/05/30 09:00
CRDT- 2009/02/03 09:00
PHST- 2008/10/15 00:00 [received]
PHST- 2009/01/08 00:00 [accepted]
PHST- 2008/12/10 00:00 [revised]
PHST- 2009/02/03 09:00 [entrez]
PHST- 2009/02/03 09:00 [pubmed]
PHST- 2009/05/30 09:00 [medline]
AID - 10.1007/s00216-009-2608-0 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2009 Mar;393(6-7):1719-31. doi: 10.1007/s00216-009-2608-0. 
      Epub 2009 Jan 31.