PMID- 19185603
OWN - NLM
STAT- MEDLINE
DCOM- 20090428
LR  - 20211020
IS  - 1873-2747 (Electronic)
IS  - 0361-9230 (Print)
IS  - 0361-9230 (Linking)
VI  - 79
IP  - 2
DP  - 2009 Apr 29
TI  - MCP-1 involvement in glial differentiation of neuroprogenitor cells through APP 
      signaling.
PG  - 97-103
LID - 10.1016/j.brainresbull.2009.01.004 [doi]
AB  - Previously it has been reported that neural stem cells undergoing apoptotic 
      stress have increased levels of amyloid precursor protein (APP) and increased APP 
      expression results in glial differentiation. APP activity was also shown to be 
      required for staurosporine-induced glial differentiation of neuroprogenitor 
      cells. Monocyte chemoattractant protein-1 (MCP-1) is a chemokine that is 
      expressed early during inflammation. The binding of MCP-1 to its chemokine 
      receptor induces expression of novel transcription factor MCP-1-induced protein 
      (MCPIP). MCPIP expression subsequently leads to cell death. Previous studies have 
      shown that pro-apoptotic factors have the ability to induce neural 
      differentiation. Therefore, we investigated if MCPIP expression leads to 
      differentiation of NT2 neuroprogenitor cells. Results showed that MCPIP 
      expression increased glial fibrillary acid protein (GFAP) expression and also 
      caused distinct morphological changes, both indicative of glial differentiation. 
      Similar results were observed with MCP-1 treatment. Interestingly, APP expression 
      decreased in response to MCPIP. Instead, we found APP activity regulates 
      expression of both MCP-1 and MCPIP. Furthermore, inhibition of either p38 MAPK or 
      JAK signaling pathways significantly reduced APP's effect on MCP-1 and MCPIP. 
      These data demonstrates the role APP has in glial differentiation of NT2 cells 
      through MCP-1/MCPIP signaling. It is possible that increased APP expression after 
      CNS injury could play a role in MCP-1 production, possibly promoting astrocyte 
      activation at injured site.
FAU - Vrotsos, Emmanuel George
AU  - Vrotsos EG
AD  - Burnett School of Biomedical Sciences, College of Medicine, University of Central 
      Florida, Orlando, FL 32816-2364, USA.
FAU - Kolattukudy, Pappachan E
AU  - Kolattukudy PE
FAU - Sugaya, Kiminobu
AU  - Sugaya K
LA  - eng
GR  - R01 AG023472/AG/NIA NIH HHS/United States
GR  - R01 AG023472-01/AG/NIA NIH HHS/United States
GR  - AG 23472/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090129
PL  - United States
TA  - Brain Res Bull
JT  - Brain research bulletin
JID - 7605818
RN  - 0 (APP protein, human)
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Protease Nexins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (STAT3 protein, human)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.10.2 (Janus Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.1.- (Ribonucleases)
RN  - EC 3.1.- (ZC3H12A protein, human)
SB  - IM
MH  - Amyloid beta-Protein Precursor/*metabolism
MH  - Cell Death/physiology
MH  - *Cell Differentiation/physiology
MH  - Cell Line
MH  - Chemokine CCL2/*metabolism
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Humans
MH  - Janus Kinases/metabolism
MH  - MAP Kinase Signaling System
MH  - Neuroglia/cytology/*physiology
MH  - Phosphorylation
MH  - Protease Nexins
MH  - RNA, Messenger/metabolism
MH  - Receptors, Cell Surface/*metabolism
MH  - Ribonucleases
MH  - STAT3 Transcription Factor/metabolism
MH  - Signal Transduction
MH  - Stem Cells/*physiology
MH  - Transcription Factors/*metabolism
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
PMC - PMC3427747
MID - NIHMS108042
EDAT- 2009/02/03 09:00
MHDA- 2009/04/29 09:00
PMCR- 2012/08/26
CRDT- 2009/02/03 09:00
PHST- 2008/08/13 00:00 [received]
PHST- 2008/12/17 00:00 [revised]
PHST- 2009/01/06 00:00 [accepted]
PHST- 2009/02/03 09:00 [entrez]
PHST- 2009/02/03 09:00 [pubmed]
PHST- 2009/04/29 09:00 [medline]
PHST- 2012/08/26 00:00 [pmc-release]
AID - S0361-9230(09)00014-8 [pii]
AID - 10.1016/j.brainresbull.2009.01.004 [doi]
PST - ppublish
SO  - Brain Res Bull. 2009 Apr 29;79(2):97-103. doi: 
      10.1016/j.brainresbull.2009.01.004. Epub 2009 Jan 29.