PMID- 19186836 OWN - NLM STAT- MEDLINE DCOM- 20091029 LR - 20161018 IS - 0250-3301 (Print) IS - 0250-3301 (Linking) VI - 29 IP - 11 DP - 2008 Nov TI - [Effect of temperature on methanogenic pathway during household waste anaerobic digestion by stable carbon isotopic signature of CH4]. PG - 3252-7 AB - The methanogenic pathway during anaerobic digestion of household waste was investigated by stable carbon isotopic signature analysis, and testified by the analysis of gas production, leachate characteristics and microbial fluorescence in situ hybridization (FISH) methods. Furthemore, the difference of methanogenic pathway between mesophilic and thermophilic anaerobic digestion was also discussed. Results showed that under mesophilic conditions, the isotopic stable carbon signature of CH4 (delta13 CH4) initially decreased to -69.5 per thousand, indicating that CH4 was produced from CO2 and H2 by hydrogenotrophic methanogens. When active CH4 production phase started, the delta13 CH4 values quickly increased to -23.8 per thousand, which indicated more and more CH4 were formed by aceticlastic methanogens, dominantly the family of Methanosarcinaceae, shown by the FISH results. The delta13 CH4 values decreased successively and ultimately remained at -55 per thousand, indicating that the fraction of aceticlastic methanogenesis finally decreased to a steady level comparative with CO2-derived methanogenesis at the steady slow methane production phase. Under thermophilic conditions, the delta13 CH4 values remained at a level about -70 per thousand, showing that methane were solely produced from CO2 reduction, and acetate syntrophic oxidation happened during the active methane production phase. FAU - Qu, Xian AU - Qu X AD - State Key Laboratory of Pollution Control and Resource Reuse, Key laboratory of Yangtze River Water Environment, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. solidwaste@mail.tongji.edu.cn FAU - He, Pin-Jing AU - He PJ FAU - Mazeas, Laurent AU - Mazeas L FAU - Bouchez, Theodore AU - Bouchez T LA - chi PT - English Abstract PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - Huan Jing Ke Xue JT - Huan jing ke xue= Huanjing kexue JID - 8405344 RN - 0 (Acetates) RN - 0 (Carbon Isotopes) RN - 142M471B3J (Carbon Dioxide) RN - OP0UW79H66 (Methane) SB - IM MH - Acetates/chemistry MH - Anaerobiosis MH - Bioreactors/*microbiology MH - Carbon Dioxide/chemistry MH - Carbon Isotopes/*analysis MH - Methane/analysis/chemistry/*metabolism MH - Methanosarcinaceae/*metabolism MH - Oxidation-Reduction MH - Refuse Disposal/*methods MH - Temperature EDAT- 2009/02/04 09:00 MHDA- 2009/10/30 06:00 CRDT- 2009/02/04 09:00 PHST- 2009/02/04 09:00 [entrez] PHST- 2009/02/04 09:00 [pubmed] PHST- 2009/10/30 06:00 [medline] PST - ppublish SO - Huan Jing Ke Xue. 2008 Nov;29(11):3252-7.