PMID- 19187610
OWN - NLM
STAT- MEDLINE
DCOM- 20090522
LR  - 20191111
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Linking)
VI  - 122
IP  - 1
DP  - 2009 Jan 5
TI  - Altered expression profiles of microRNAs in a stable hepatitis B virus-expressing 
      cell line.
PG  - 10-4
AB  - BACKGROUND: MicroRNAs (miRNAs) are highly conserved small non-coding RNAs of 18 - 
      25 nucleotides (nt) that mediate post-transcriptional gene regulation. Hepatitis 
      B virus (HBV) can cause either acute or chronic hepatitis B, and is a high risk 
      factor for liver cirrhosis and hepatocellular carcinoma. Some mammalian viruses 
      have been shown to modulate the expression of host cellular miRNAs. However, 
      interactions between the HBV and the host cellular miRNAs are largely unknown. 
      METHODS: miRNA microarray and Northern blotting analysis were used to compare the 
      expression profile of cellular miRNAs of a stable HBV-expressing cell line 
      HepG2.2.15 and its parent cell line HepG2. mRNA microarray assay and the miRanda 
      program were used to predict the miRNA targets. A flow cytometric assay was 
      further used to investigate the expression of human leukocyte antigen (HLA)-A. 
      RESULTS: Eighteen miRNAs were differentially expressed between the two cell 
      lines. Among them, eleven were up-regulated and seven were down-regulated in 
      HepG2.2.15 cells. Northern blotting analysis confirmed that the expression of 
      miR-181a, miR-181b, miR-200b and miR-146a were up-regulated and the expression of 
      miR-15a was down-regulated, which was in consistent with the results of the 
      microarray analysis. Furthermore, some putative miRNA targets were predicted and 
      verified to be linked with mRNA expression. The 3'-UTR of HLA-A gene had one 
      partially complementary site for miR-181a and miR-181a might down-regulate the 
      expression of HLA-A. CONCLUSION: HBV replication modulates the expression of host 
      cellular miRNAs, which may play a role in the pathogenesis of HBV-related liver 
      diseases.
FAU - Liu, Yan
AU  - Liu Y
AD  - Viral Hepatitis Research Laboratory, Institute of Infectious Diseases, Beijing 
      Hospital, Beijing, China.
FAU - Zhao, Jian-Jun
AU  - Zhao JJ
FAU - Wang, Chun-mei
AU  - Wang CM
FAU - Li, Mian-yang
AU  - Li MY
FAU - Han, Ping
AU  - Han P
FAU - Wang, Lin
AU  - Wang L
FAU - Cheng, Yong-qian
AU  - Cheng YQ
FAU - Zoulim, Fabien
AU  - Zoulim F
FAU - Ma, Xu
AU  - Ma X
FAU - Xu, Dong-ping
AU  - Xu DP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (HLA-A Antigens)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Blotting, Northern
MH  - Cell Line, Tumor/*metabolism/*virology
MH  - Flow Cytometry
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - HLA-A Antigens/*metabolism
MH  - Hepatitis B virus/*growth & development/physiology
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Oligonucleotide Array Sequence Analysis
EDAT- 2009/02/04 09:00
MHDA- 2009/05/23 09:00
CRDT- 2009/02/04 09:00
PHST- 2009/02/04 09:00 [entrez]
PHST- 2009/02/04 09:00 [pubmed]
PHST- 2009/05/23 09:00 [medline]
AID - 10.3901/jme.2009.11.010 [doi]
PST - ppublish
SO  - Chin Med J (Engl). 2009 Jan 5;122(1):10-4. doi: 10.3901/jme.2009.11.010.