PMID- 19189060
OWN - NLM
STAT- MEDLINE
DCOM- 20090923
LR  - 20131121
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 158
IP  - 2
DP  - 2009 Aug
TI  - Rapid screening and isolation of a fungus for sitosterol to androstenedione 
      biotransformation.
PG  - 374-86
LID - 10.1007/s12010-008-8416-8 [doi]
AB  - Androstenedione (AD) is a steroid intermediate used in the pharmaceutical 
      industry for the production of several important anabolic drugs. An important 
      route for producing AD is by the microbial transformation of sterols. Microbes 
      capable of efficiently transforming sterols to AD are few and newer strains need 
      to be isolated. Conventional procedures for screening and isolation are time 
      consuming. A new procedure was used for screening and isolation of fungal 
      microorganisms capable of biotransformation of sitosterol to androstenedione. In 
      this procedure, Basic Alignment Search Tool (BLAST) and Position-Specific 
      Iterative BLAST were employed to obtain a parent set of candidate microorganisms. 
      The parent set was reduced using heuristics and constraints to obtain a 
      manageable number of microorganisms that may be tested experimentally. For this 
      work, screening of the entire NCBI database yielded a parent set containing 64 
      microorganisms. Among these, only two microorganisms, Aspergillus oryzae and 
      Aspergillus nidulans FGSC A4, qualified to the experimental stage. Sitosterol 
      biotransformation experiments were carried out using A. oryzae and the production 
      of AD in culture medium was confirmed.
FAU - Malaviya, Alok
AU  - Malaviya A
AD  - Department of Biochemical Engineering & Biotechnology, Indian Institute of 
      Technology Delhi, Hauz Khas, New Delhi 110016, India.
FAU - Gomes, James
AU  - Gomes J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090203
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Sitosterols)
RN  - 409J2J96VR (Androstenedione)
RN  - 5LI01C78DD (gamma-sitosterol)
SB  - IM
MH  - Androstenedione/chemistry/*metabolism
MH  - Aspergillus nidulans/metabolism
MH  - Aspergillus oryzae/metabolism
MH  - Chromatography, Thin Layer
MH  - Computational Biology
MH  - Fungi/*metabolism
MH  - Molecular Structure
MH  - Sitosterols/chemistry/*metabolism
EDAT- 2009/02/04 09:00
MHDA- 2009/09/24 06:00
CRDT- 2009/02/04 09:00
PHST- 2008/05/28 00:00 [received]
PHST- 2008/10/27 00:00 [accepted]
PHST- 2009/02/04 09:00 [entrez]
PHST- 2009/02/04 09:00 [pubmed]
PHST- 2009/09/24 06:00 [medline]
AID - 10.1007/s12010-008-8416-8 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2009 Aug;158(2):374-86. doi: 10.1007/s12010-008-8416-8. 
      Epub 2009 Feb 3.