PMID- 19189219
OWN - NLM
STAT- MEDLINE
DCOM- 20091013
LR  - 20211020
IS  - 1557-1904 (Electronic)
IS  - 1557-1890 (Linking)
VI  - 4
IP  - 3
DP  - 2009 Sep
TI  - Inducible expression of functional mu opioid receptors in murine dendritic cells.
PG  - 359-67
LID - 10.1007/s11481-009-9145-7 [doi]
AB  - Opioids are known to exert direct effects on the immune system, and the 
      expression of functional opioid receptors has been reported on several immune 
      cell types. Dendritic cells (DCs) are important inducers and regulators of immune 
      responses. In this study, we investigated whether murine dendritic cells express 
      functional mu opioid receptors (MOR). RT-PCR analysis and double 
      immunofluorescence staining revealed the expression of MOR in activated murine 
      dendritic cells. We also studied the dynamic expression of MOR messenger RNA in 
      murine dendritic cells in response to different Toll-like receptor ligands. 
      Functionally, treatment of DCs with endomorphin 1 (EM1), a specific agonist of 
      MOR, can inhibit the forskolin-induced formation of cyclic adenosine 
      monophosphate level in activated DCs. Moreover, EM1 treatment resulted in less 
      activation of p38 MAPK and more activation of ERK signaling in 
      lipopolysaccharide-stimulated DCs. Consistently, treatment of DCs with EM1 
      altered cytokine production by increasing IL-10 and decreasing IL-12 and IL-23. 
      Our results suggest that MOR is inducibly expressed on activated DCs and 
      functionally mediates EM1-induced effects on DCs. Thus, dendritic cells might be 
      involved in crosstalk between the neuroendocrine and the immune system.
FAU - Li, Zheng-Hong
AU  - Li ZH
AD  - Department of Neurobiology, School of Medicine, Soochow University, Suzhou 
      215123, People's Republic of China.
FAU - Chu, Niansheng
AU  - Chu N
FAU - Shan, Li-Dong
AU  - Shan LD
FAU - Gong, Shan
AU  - Gong S
FAU - Yin, Qi-Zhang
AU  - Yin QZ
FAU - Jiang, Xing-Hong
AU  - Jiang XH
LA  - eng
PT  - Journal Article
DEP - 20090203
PL  - United States
TA  - J Neuroimmune Pharmacol
JT  - Journal of neuroimmune pharmacology : the official journal of the Society on 
      NeuroImmune Pharmacology
JID - 101256586
RN  - 0 (Analgesics, Opioid)
RN  - 0 (Interleukin-23)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Oligopeptides)
RN  - 0 (Receptors, Opioid, mu)
RN  - 0 (endomorphin 1)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 1F7A44V6OU (Colforsin)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Analgesics, Opioid/pharmacology
MH  - Animals
MH  - Blotting, Western
MH  - Bone Marrow Cells/drug effects/metabolism
MH  - Colforsin/pharmacology
MH  - Cyclic AMP/biosynthesis
MH  - Dendritic Cells/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Interleukin-10/biosynthesis
MH  - Interleukin-12/biosynthesis
MH  - Interleukin-23/biosynthesis
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Confocal
MH  - Oligopeptides/pharmacology
MH  - Receptors, Opioid, mu/agonists/*biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
EDAT- 2009/02/04 09:00
MHDA- 2009/10/14 06:00
CRDT- 2009/02/04 09:00
PHST- 2008/06/14 00:00 [received]
PHST- 2009/01/06 00:00 [accepted]
PHST- 2009/02/04 09:00 [entrez]
PHST- 2009/02/04 09:00 [pubmed]
PHST- 2009/10/14 06:00 [medline]
AID - 10.1007/s11481-009-9145-7 [doi]
PST - ppublish
SO  - J Neuroimmune Pharmacol. 2009 Sep;4(3):359-67. doi: 10.1007/s11481-009-9145-7. 
      Epub 2009 Feb 3.